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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Karyotype characterization of two populations of Vernonia geminata (Asteraceae, Vernonieae) using banding and FISH techniques

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Author(s):
Oliveira, V. M. [1] ; Mansanares, M. E. [2] ; Semir, J. [1] ; Forni-Martins, E. R. [1]
Total Authors: 4
Affiliation:
[1] Univ Estadual Campinas, Lab Biossistemat & Evolucao, Dept Biol Vegetal, Inst Biol, Campinas, SP - Brazil
[2] Univ Fed Lavras, Setor Bot, Dept Biol, Lavras, MG - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Genetics and Molecular Research; v. 11, n. 4, p. 4204-4212, 2012.
Web of Science Citations: 1
Abstract

In order to extend our knowledge concerning karyotypes of the genus Vernonia, we applied various techniques of chromosome banding, including AgNOR and triple staining with the fluorochromes CMA/DA/DAPI (CDD), and of fluorescent in situ hybridization (FISH) for the 45S rDNA probe to specimens of two populations of Vernonia geminata collected from an open-pasture area, in southern Brazil. B chromosomes were observed in one of the populations. Both populations of V. geminata presented a pair of CMA(3)(+) terminal bands and one pair of chromosomes with terminal AgNOR banding. The FISH evidenced, in one population, two pairs of small sites of 45S rDNA; these being two small terminal sites and two centromeric sites. In the other population, there was only one pair of small terminal sites and two sites in two B chromosomes, one in each chromosome. There was coincidence of localization between CMA(+) and NOR bands with one of the small terminal sites of 45S rDNA of one chromosome of the normal complement, but not in B chromosomes. (AU)