A homolog of the RPS2 disease resistance gene is constitutively expressed in Brassica oleracea

Autor(es)

Celia C. Malvas ^[1] ; Maeli Melotto ^[2] ; Daniela Truffi ^[3] ; Luis E.A. Camargo ^[4]

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. ESALQ. Departamento de Patologia de Plantas - Brasil

^[2] Universidade de São Paulo. ESALQ. Departamento de Patologia de Plantas - Brasil

^[3] Universidade de São Paulo. ESALQ. Departamento de Patologia de Plantas - Brasil

^[4] Universidade de São Paulo. ESALQ. Departamento de Patologia de Plantas - Brasil

• Tipo de documento: Artigo Científico
• Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 26, n. 4, p. 511-516, 2003-12-00.

Resumo

In this study, we identified disease resistance gene homologs in Brassica oleracea and assessed their expression in lines resistant and susceptible to Xanthomonas campestris pv. campestris (Xcc). Two DNA fragments of approximately 2.5 kb (BI-16/RPS2 and Lc201/RPS2) were amplified by PCR from two Brassica lines using primers based on an RPS2 homologous sequence previously described in the Brassica oleracea ecotype B117. The sequences of these fragments shared high similarity (95-98%) with RPS2 homologs from various Brassica species. The digestion of these fragments with restriction enzymes revealed polymorphisms at the Xba I restriction sites. The length polymorphisms were used as a co-dominant marker in an F2 population developed to segregate for resistance to Xcc, the causal agent of black rot. Linkage analysis showed no significant association between the marker and quantitative trait loci for black rot. RT-PCR with specific primers yielded an expected 453 bp fragment that corresponded to the RPS2 homologs in both resistant and susceptible lines inoculated with the pathogen, as well as in non-inoculated control plants. These results suggest that these homologs are constitutively expressed in B. oleracea. (AU)

• Processo FAPESP: 99/00800-8 - Clonagem e caracterização genética de locos homólogos a genes de resistência em Zea mays e Brassica oleracea
• Beneficiário: Celia Correia Malvas
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 00/09059-8 - Agronomical and environmental genomes
• Beneficiário: Luis Eduardo Aranha Camargo
• Modalidade de apoio: Auxílio à Pesquisa - Programa GENOMA