Topical application of the lectin Artin M accelerates wound healing in rat oral mucosa by enhancing TGF-beta and VEGF production

Autor(es)

Kim, Yeon J. ^[1] ; Carvalho, Fernanda C. ^[2] ; Souza, Joao A. C. ^[1] ; Goncalves, Pedro C. G. ^[1] ; Nogueira, Andressa V. B. ^[1] ; Spolidorio, Luis C. ^[1] ; Roque-Barreira, Maria C. ^[2] ; Cirelli, Joni A. ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Dept Diag & Surg, Div Periodontol, Sch Dent, BR-14801903 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Cellular & Mol Biol, Sch Med Ribeirao Preto, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: WOUND REPAIR AND REGENERATION; v. 21, n. 3, p. 456-463, MAY-JUN 2013.
• Citações Web of Science: 13

Resumo

The lectin Artin M has been shown to accelerate the wound-healing process. The aims of this study were to evaluate the effects of Artin M on wound healing in the palatal mucosa of rats and to investigate the effects of Artin M on transforming growth factor beta (TGF-) and vascular endothelial growth factor (VEGF) secretion by rat gingival fibroblasts. A surgical wound was created on the palatal mucosa of 72 rats divided into three groups according to treatment: CControl (nontreated), AArtin M gel, and VVehicle. Eight animals per group were sacrificed at 3, 5, and 7 days postsurgery for histology, immunohistochemistry and determination of the levels of cytokines, and growth factors. Gingival fibroblasts were incubated with 2.5g/mL of Artin M for 24, 48, and 72 hours. The expression of VEGF and TGF- was determined by enzyme-linked immunosorbent assay. Histologically, at day 7, the Artin M group showed earlier reepithelialization, milder inflammatory infiltration, and increased collagen fiber formation, resulting in faster maturation of granular tissue than in the other groups (p<0.05). Artin Minduced cell proliferation in vivo and promoted a greater expression of TGF- and VEGF in both experiments (p<0.05). Artin M was effective in healing oral mucosa wounds in rats and was associated with increased TGF- and VEGF release, cell proliferation, reepithelialization, and collagen deposition and arrangement of fibers. (AU)

• Processo FAPESP: 09/16432-1 - Avaliação do efeito da Artin M no processo de reparação em mucosa mastigatória: estudo in vivo e in vitro
• Beneficiário: Joni Augusto Cirelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/60642-2 - Efeitos biológicos e aplicações farmacêuticas de lectinas
• Beneficiário: Maria Cristina Roque Antunes Barreira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 09/16955-4 - Avaliação do efeito do gel de artin m no processo de reparação em mucosa mastigatória de ratos
• Beneficiário: Pedro César Garcia Gonçalves
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 09/03032-5 - Efeito de Artin M sobre o curso da leucemia promielocítica aguda desenvolvida em modelos murinos: distinção entre ações anti-tumoral e imunomoduladora. Projeto Temático: Efeitos biológicos e aplicações farmacêuticas de lectinas.
• Beneficiário: Fernanda Caroline de Carvalho
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado