Photodynamic inactivation of Streptococcus mutans and Streptococcus sanguinis biofilms in vitro

Autor(es)

Pereira, Cristiane Aparecida ^[1] ; Borges Pereira Costa, Anna Carolina ^[1] ; Carreira, Claudia Moura ^[2] ; Junqueira, Juliana Campos ^[1] ; Cardoso Jorge, Antonio Olavo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Sch Dent Sao Jose dos Campos, Dept Biosci & Oral Diag, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[2] Escola Super Amazonia ESAMAZ, Dept Dent, BR-66023570 Belem, PA - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 28, n. 3, p. 859-864, MAY 2013.
• Citações Web of Science: 32

Resumo

The purpose of this study was to evaluate specific effects of photodynamic inactivation (PDI) using erythrosine (ER) and Rose Bengal (RB) photosensitizers and a blue light-emitting diode (LED) on the viability of Streptococcus mutans and Streptococcus sanguinis biofilms. Biofilms were grown in acrylic disks immersed in broth to production of biofilms, inoculated with microbial suspension (10(6) cells/mL) and incubated for 48 h. After the formation of biofilms, the effects of the photosensitizers ER and RB at a concentration of 5 mu M for 5 min and blue LED (455 +/- 20 nm) for 180 s, photosensitizers alone and conjugated were evaluated. Next, the disks were placed in tubes with sterile physiological solution (0.9 % sodium chloride) and sonicated for to disperse the biofilms. Tenfold serial dilutions were carried and aliquots seeded in brain heart infusion agar which were then incubated for 48 h. Then the numbers colony-forming units per milliliter (CFU/mL; log(10)) were counted and analyzed statistically (ANOVA, Tukey test, P a parts per thousand currency sign 0.05). Significant decreases in the viability of all microorganisms were observed for biofilms exposed to PDI mediated by both photosensitizers. The reductions with RB and ER were, 0.62 and 0.52 log(10) CFU mL(-1) for S. mutans biofilms (p = 0.001), and 0.95 and 0.88 log(10) CFU mL(-1) for S. sanguinis biofilms (p = 0.001), respectively. The results showed that biofilms formed in vitro by S. mutans and S. sanguinis, were sensitive to PDI using a blue LED associated with photosensitizers ER or RB, indicating its use in the control of caries and periodontal diseases. (AU)

• Processo FAPESP: 10/00879-4 - MICRORGANISMOS OPORTUNISTAS EM LESÕES DE ESTOMATITE PROTÉTICA E INFLUÊNCIA DA TERAPIA FOTODINÂMICA NOS FATORES DE VIRULÊNCIA DE Candida spp.
• Beneficiário: Cristiane Aparecida Pereira Correia
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/21346-7 - GENES DE Candida albicans COM FUNÇÃO DESCONHECIDA QUANTO À FORMAÇÃO DE BIOFILME, CARACTERÍSTICAS BIOLÓGICAS E INTERAÇÃO PATÓGENO-HOSPEDEIRO
• Beneficiário: Anna Carolina Borges Pereira da Costa
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/52048-1 - Efeitos da terapia fotodinâmica e de óleos essenciais de Cymbopogon citratus (DC.) Stapf, Casearia sylvestris SW e Curcuma zedoaria Roscoe, in vitro, em biofilmes formados com Candida albicans, Staphylococcus aureus e Streptococcus mutans
• Beneficiário: Antonio Olavo Cardoso Jorge
• Modalidade de apoio: Auxílio à Pesquisa - Regular