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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Photodynamic inactivation of Streptococcus mutans and Streptococcus sanguinis biofilms in vitro

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Author(s):
Pereira, Cristiane Aparecida [1] ; Borges Pereira Costa, Anna Carolina [1] ; Carreira, Claudia Moura [2] ; Junqueira, Juliana Campos [1] ; Cardoso Jorge, Antonio Olavo [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Paulista UNESP, Sch Dent Sao Jose dos Campos, Dept Biosci & Oral Diag, BR-12245000 Sao Jose Dos Campos, SP - Brazil
[2] Escola Super Amazonia ESAMAZ, Dept Dent, BR-66023570 Belem, PA - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Lasers in Medical Science; v. 28, n. 3, p. 859-864, MAY 2013.
Web of Science Citations: 32
Abstract

The purpose of this study was to evaluate specific effects of photodynamic inactivation (PDI) using erythrosine (ER) and Rose Bengal (RB) photosensitizers and a blue light-emitting diode (LED) on the viability of Streptococcus mutans and Streptococcus sanguinis biofilms. Biofilms were grown in acrylic disks immersed in broth to production of biofilms, inoculated with microbial suspension (10(6) cells/mL) and incubated for 48 h. After the formation of biofilms, the effects of the photosensitizers ER and RB at a concentration of 5 mu M for 5 min and blue LED (455 +/- 20 nm) for 180 s, photosensitizers alone and conjugated were evaluated. Next, the disks were placed in tubes with sterile physiological solution (0.9 % sodium chloride) and sonicated for to disperse the biofilms. Tenfold serial dilutions were carried and aliquots seeded in brain heart infusion agar which were then incubated for 48 h. Then the numbers colony-forming units per milliliter (CFU/mL; log(10)) were counted and analyzed statistically (ANOVA, Tukey test, P a parts per thousand currency sign 0.05). Significant decreases in the viability of all microorganisms were observed for biofilms exposed to PDI mediated by both photosensitizers. The reductions with RB and ER were, 0.62 and 0.52 log(10) CFU mL(-1) for S. mutans biofilms (p = 0.001), and 0.95 and 0.88 log(10) CFU mL(-1) for S. sanguinis biofilms (p = 0.001), respectively. The results showed that biofilms formed in vitro by S. mutans and S. sanguinis, were sensitive to PDI using a blue LED associated with photosensitizers ER or RB, indicating its use in the control of caries and periodontal diseases. (AU)

FAPESP's process: 10/00879-4 - OPPORTUNISTIC MICROORGANISMS IN INJURIES denture stomatitis AND INFLUENCE OF PHOTODYNAMIC THERAPY IN VIRULENCE FACTORS OF Candida spp.
Grantee:Cristiane Aparecida Pereira Correia
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 11/21346-7 - GENES WITH UNKNOWN FUNCTION OF Candida albicans AS TO BIOFILM FORMATION, BIOLOGICAL CHARACTERISTICS AND HOST- PATHOGEN INTERACTION
Grantee:Anna Carolina Borges Pereira da Costa
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 09/52048-1 - Effects of photodynamic therapy and essential oils of the Cymbopogon citratus (DC.) Stapf, Casearia sylvestris SW and Curcuma zedoaria Roscoe, in vitro, in biofilms of Candida albicans, Staphylococcus aureus and Streptococcus mutans
Grantee:Antonio Olavo Cardoso Jorge
Support Opportunities: Regular Research Grants