In vitro Leishmanicidal and Cytotoxic Activities of the Glycoalkaloids from Solanum lycocarpum (Solanaceae) Fruits

Autor(es):Mostrar menos -

Miranda, Mariza Abreu ^[1] ; Jorge Tiossi, Renata Fabiane ^[1] ; da Silva, Mariana Rosa ^[1] ; Rodrigues, Kelly Cristina ^[1] ; Kuehn, Christian Collins ^[1] ; Rodrigues Oliveira, Luiz Gustavo ^[1] ; Albuquerque, Sergio ^[1] ; McChesney, James Dewey ^[2] ; Lezama-Davila, Claudio Manuel ^[3] ; Isaac-Marquez, Angelica Patricia ^[3] ; Bastos, Jairo Kenupp ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Sao Paulo - Brazil

^[2] Arbor Therapeut LLC, Etta, MS 38627 - USA

^[3] Ohio State Univ, Dept Pathol, OSU Med Ctr, Columbus, OH 43210 - USA

• Tipo de documento: Artigo Científico
• Fonte: CHEMISTRY & BIODIVERSITY; v. 10, n. 4, p. 642-648, APR 2013.
• Citações Web of Science: 17

Resumo

Leishmaniasis is an infection caused by a protozoan parasite of the genus Leishmania and is the second most prevalent parasitic protozoal disease after malaria in the world. We report the in vitro leishmanicidal activity on promastigote forms of Leishmania amazonensis and cytotoxicity, using LLCMK2 cells, of the glycoalkaloids from the fruits of Solanum lycocarpum, determined by colorimetric methods. The alkaloidic extract was obtained by acid-base extraction; solamargine and solasonine were isolated by silica-gel chromatography, followed by reversed-phase HPLC final purification. The alkaloidic extract, solamargine, solasonine, as well as the equimolar mixture of the glycoalkaloids solamargine and solasonine displayed leishmanicidal activity against promastigote forms of L. amazonensis, whereas the aglycone solasodine was inactive. After 24 and 72h of incubation, most of the samples showed lower cytotoxicities (IC50 6.5 to 124M) as compared to leishmanicidal activity (IC50 1.1 to 23.6M). The equimolar mixture solamargine/solasonine was the most active with an IC50 value of 1.1M, after 72h. Likewise, solamargine was the most active after 24h with an IC50 value of 14.4M, both in comparison with the positive control amphotericin B. (AU)

• Processo FAPESP: 08/56324-0 - Avaliacao do potencial antiparasitario dos glicoalcaloides dos frutos de solanum lycocarpum.
• Beneficiário: Mariza Abreu Miranda
• Modalidade de apoio: Bolsas no Brasil - Mestrado