Trafficking of phagocytic peritoneal cells in hypoinsulinemic-hyperglycemic mice with systemic candidiasis

Autor(es)

de Campos Fraga-Silva, Thais Fernanda ^{[1, 2]} ; Venturini, James ^{[1, 3]} ; Parreira de Arruda, Maria Sueli ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Fac Ciencias, Dept Ciencias Biol, Lab Imunopatol Expt LIPE, BR-17033360 Bauru, SP - Brazil

^[2] UNESP Univ Estadual Paulista, Inst Biociencias, BR-18618970 Botucatu, SP - Brazil

^[3] UNESP Univ Estadual Paulista, Fac Med Botucatu, BR-18618970 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC INFECTIOUS DISEASES; v. 13, MAR 25 2013.
• Citações Web of Science: 6

Resumo

Background: Candidemia is a severe fungal infection that primarily affects hospitalized and/or immunocompromised patients. Mononuclear phagocytes have been recognized as pivotal immune cells which act in the recognition of pathogens, phagocytosis, inflammation, polarization of adaptive immune response and tissue repair. Experimental studies have showed that the systemic candidiasis could be controlled by activated peritoneal macrophages. However, the mechanism to explain how these cells act in distant tissue during a systemic fungal infection is still to be elucidated. In the present study we investigate the in vivo trafficking of phagocytic peritoneal cells into infected organs in hypoinsulinemic-hyperglycemic (HH) mice with systemic candidiasis. Methods: The red fluorescent vital dye PKH-26 PCL was injected into the peritoneal cavity of Swiss mice 24 hours before the intravenous inoculation with Candida albicans. After 24 and 48 hours and 7 days of infection, samples of the spleen, liver, kidneys, brain and lungs were submitted to the microbiological evaluation as well as to phagocytic peritoneal cell trafficking analyses by fluorescence microscopy. Results: In the present study, PKH+ cells were observed in the peritoneum, kidney, spleen and liver samples from all groups. In infected mice, we also found PKH+ cells in the lung and brain. The HH condition did not affect this process. Conclusions: In the present study we have observed that peritoneal phagocytes migrate to tissues infected by C. albicans and the HH condition did not interfere in this process. (AU)

• Processo FAPESP: 09/50818-4 - Estudo do estado de ativação de macrófagos peritoneais e sua relação com a evolução de lesões subcutâneas: possível tráfego de células entre a cavidade peritoneal e o tecido comprometido
• Beneficiário: Thais Fernanda de Campos Fraga da Silva
• Linha de fomento: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 09/50654-1 - Contribuição ao estudo da dermatofitose experimental: determinação das subpopulações de monócitos e macrófagos em camundongos HH infectados com Trichophyton mentagrophyte
• Beneficiário: Maria Sueli Parreira de Arruda
• Linha de fomento: Auxílio à Pesquisa - Regular