Rat Embryonic Mast Cells Originate in the AGM

Autor(es)

Guiraldelli, Michel Farchi ^[1] ; Franca, Carolina Nunes ^[1] ; de Souza, Jr., Devandir Antonio ^[1] ; Marcelino da Silva, Elaine Zayas ^[1] ; Toso, Vanina Danuza ^[1] ; Carvalho, Celiane Cardoso ^[1] ; Jamur, Maria Celia ^[1] ; Oliver, Constance ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Cell & Mol Biol & Pathogen Bioagents, BR-14049 Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 8, n. 3 MAR 7 2013.
• Citações Web of Science: 5

Resumo

Mast cells originate from pluripotent hematopoietic stem cells. Two mast cell specific antibodies, mAbsAA4 and BGD6, have previously been used to identify and study committed mast cell precursors (MCcps) in the bone marrow of adult mice and rats. However, the embryonic origin of MCcps is still not known. In the present study, we identified MCcps in rat embryos using these previously characterized mast cell specific antibodies. The MCcps were found in the AGM (aorta-gonad-mesonephros) region of rat embryos at E11.5. These cells were BGD6+, CD34(+), c-kit(+), CD13(+), Fc epsilon RI-, AA4(-) CD40(-), and Thy-1(-). By PCR the cells contained message for the alpha and beta subunits of Fc epsilon RI and mast cell specific proteases. In vitro, the MCcps differentiated into metachromatic mast cells. With age of gestation the percent of MCcps diminished while the percent of mast cell progenitors increased. An increased knowledge of the biology and embryonic origin of mast cells may contribute to a greater understanding of allergy, asthma, and other mast cell related diseases. (AU)

• Processo FAPESP: 03/00543-2 - Isolamento e caracterizacao de precursores da linhagem de mastocitos de embrioes de rato
• Beneficiário: Maria Célia Jamur
• Modalidade de apoio: Auxílio à Pesquisa - Regular