Development of poly(lactic acid) nanostructured membranes for the controlled delivery of progesterone to livestock animals

Autor(es)

Oliveira, Juliano Elvis ^[1] ; Medeiros, Eliton Souto ^[2] ; Cardozo, Lucio ^[3] ; Voll, Fernando ^[3] ; Madureira, Ed Hoffmann ^[4] ; Capparelli Mattoso, Luiz Henrique ^[1] ; Garrido Assis, Odilio Benedito ^[1]

Afiliação do(s) autor(es)

^[1] Embrapa Instrumentacao CNPDIA, LNNA, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Fed Paraiba UFPB, Dept Engn Mat DEMAT, BR-58051900 Joao Pessoa, Paraiba - Brazil

^[3] Univ Estadual Maringa, Dept Engn Quim, BR-87020900 Maringa, Parana - Brazil

^[4] Univ Sao Paulo, FMVZ, Dept Anim Reprod, Pirassununga, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 33, n. 2, p. 844-849, MAR 1 2013.
• Citações Web of Science: 23

Resumo

Solution blow spinning (SBS) is a novel technology feasible to produce nanostructured polymeric membranes loaded with active agents. In the present study, nanofibrous mats of poly(lactic acid) (PLA) loaded with progesterone (P4) were produced by SBS at different P4 concentrations. The spun membranes were characterized by scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and Fourier-transform infrared spectroscopy (FTIR). The in vitro releasing of P4 was evaluated using high-performance liquid chromatography (HPLC). Interactions between progesterone and PLA were confirmed by rheological measurements of the PLA/P4 solutions and in the spun mats by microscopy (SEM), thermal (DSC) and spectral (FTIR) analyses. SEM micrographs provided evidences of a smooth and homogeneous structure for nanostructured membranes without progesterone crystals on fiber surface. FIR spectroscopy indicated miscibility and interaction between the ester of PLA and the ketone groups of the P4 in the nanofibers. X-ray analysis indicated that the size of PLA crystallites increased with progesterone content. Finally, by in vitro release experiments it was possible to observe that the progesterone releasing follows nearly first-order kinetics, probably due to the diffusion of hormone into PLA nanofibers. (c) 2012 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 10/19860-1 - Desenvolvimento de membranas biopoliméricas nanoestruturadas para liberação controlada de hormônios e semioquímicos
• Beneficiário: Juliano Elvis de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado