Temporal and Behavioral Variability in Cannabinoid Receptor Expression in Outbred Mice Submitted to Ethanol-Induced Locomotor Sensitization Paradigm

Autor(es)

Coelhoso, Cassia C. ^[1] ; Engelke, Douglas S. ^[1] ; Filev, Renato ^[1] ; Silveira, Dartiu X. ^[2] ; Mello, Luiz E. ^[1] ; Santos-Junior, Jair G. ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Neurobiol Lab, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Psychiat & Med Psychol, Sao Paulo - Brazil

^[3] Fac Ciencias Med Santa Casa, Dept Physiol Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH; v. 37, n. 9, p. 1516-1526, SEP 2013.
• Citações Web of Science: 12

Resumo

Background There is a close relationship between the endocannabinoid system and alcoholism. This study investigated possible differential expression of cannabinoid receptors CB1 (CB1R) and CB2 (CB2R) in an outbred mice strain displaying behavioral variability to ethanol (EtOH)-induced locomotor sensitization. Methods Male adult Swiss mice treated chronically with EtOH (2g/kg, i.p., daily for 21days) were classified as EtOH\_High or EtOH\_Low according to their locomotor activity after the 21st EtOH injection. A control group was similarly injected with saline. Temporal analysis of CB1R and CB2R immunoreactivity was performed in 3 different occasions: (i) at the end of chronic EtOH treatment, (ii) on the fifth day of EtOH withdrawal, and (iii) after EtOH challenge. Results Overall, no differences were seen between experimental groups regarding the CB1R at the end of acquisition. However, there were decreases in CB2R in the prefrontal cortex and the hippocampus in EtOH\_Low mice. On the fifth day of withdrawal, only EtOH\_High mice presented increase in CB1R. Nonetheless, CB2R up-regulation was observed in both EtOH\_High and EtOH\_Low mice. EtOH challenge counteracted CB1R and CBR2 up-regulation, mainly in the EtOH\_High, in structures related to emotionality, such as prefrontal cortex, ventral tegmental area, amygdala, striatum, and hippocampus. Conclusions There are different patterns of cannabinoid receptor expression during locomotor sensitization paradigm, at both temporal and behavioral perspectives. We hypothesize that CB2R down-regulation might be related to resilience to develop locomotor sensitization, while CB1R up-regulation relates to withdrawal aspects in sensitized mice. (AU)

• Processo FAPESP: 09/16305-0 - Participação da PKM sobre as influências contextuais na sensibilização locomotora induzida pela anfetamina
• Beneficiário: Douglas Senna Engelke
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 09/16307-2 - Efeitos comportamental, neuroquímico e molecular do canabidiol em camundongos submetidos a sensibilização locomotora induzida pelo etanol
• Beneficiário: Renato Filev
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 11/03184-0 - Mecanismos epigenéticos entre pCREB e GLUR2 no modelo da sensibilização locomotora induzida pelo etanol em camundongos
• Beneficiário: Cássia de Canha Coelhoso
• Modalidade de apoio: Bolsas no Brasil - Mestrado