Nanoparticles of Lyotropic Liquid Crystals: A Novel Strategy for the Topical Delivery of a Chlorin Derivative for Photodynamic Therapy of Skin Cancer

Autor(es):Mostrar menos -

Petrilli, Raquel ^[1] ; Praca, Fabiola S. G. ^[1] ; Carollo, Aline Regina H. ^[1] ; Medina, Wanessa S. G. ^[1] ; de Oliveira, Kleber Thiago ^[2] ; Fantini, Marcia C. A. ^[3] ; Neves, Maria da Graca P. M. S. ^{[4, 5]} ; Cavaleiro, Jose A. S. ^{[4, 5]} ; Serra, Osvaldo A. ^[6] ; Iamamoto, Yassuko ^[6] ; Bentley, Maria Vitoria L. B. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Quim, BR-13560 Sao Carlos, SP - Brazil

^[3] Univ Sao Paulo, Inst Fis, BR-14040903 Ribeirao Preto, SP - Brazil

^[4] Univ Aveiro, Dept Chem, Aveiro - Portugal

^[5] Univ Aveiro, QOPNA, Aveiro - Portugal

^[6] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CURRENT NANOSCIENCE; v. 9, n. 4, p. 434-441, AUG 2013.
• Citações Web of Science: 15

Resumo

Nanoparticles of lyotropic liquid crystals loaded with a new photosensitizer (a chlorin derivative) were developed for use in photodynamic therapy (PDT). These systems were characterized by spectrofluorimetric, dynamic light scattering, and small angle X-ray diffraction (SAXRD) analyses. In vitro and in vivo penetration studies in animal models were performed using animal model membranes. The systems had a particle size of 161 +/- 4 nm and a polydispersity index of 0.175 +/- 0.027. Furthermore, SAXRD studies demonstrated that the preparations remained in the liquid crystalline phase type hexagonal after drug loading. The encapsulation rate was higher than 50%, and cell viability studies revealed that the nanodispersion is not harmful for L929 skin cells. In vitro and in vivo penetration studies confirmed that the nanodispersion of hexagonal phase enabled a higher drug skin uptake compared to the control. Additionally, a fluorescence microscopy study demonstrated a higher biodistribution of the chlorin derivative in the skin layers of hairless mice compared to the control. Taken together, the results show the potential of this nanodispersion for the delivery of the photosensitizer into the skin, which is a crucial condition for successful topical PDT. (AU)

• Processo FAPESP: 04/09465-7 - Laboratório multiusuário de caracterização de sistemas de liberação micro - e nanodispersos de fármacos
• Beneficiário: Maria Vitória Lopes Badra Bentley
• Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 09/00009-2 - Síntese e caracterização de novos sistemas porfirínicos e de macrociclos relacionados para o estudo de propriedades catalíticas e de fotossensibilizadores
• Beneficiário: Yassuko Iamamoto
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/06619-4 - Síntese de novos fotossensibilizadores com potencial aplicação em terapia fotodinâmica
• Beneficiário: Kleber Thiago de Oliveira
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores