Search for mutations in signaling pathways in head and neck squamous cell carcinoma

Autor(es)

De Carvalho, Thais Gulim ^[1] ; De Carvalho, Ana Carolina ^[1] ; Campelo Maia, Danielle Calheiros ^[1] ; Ogawa, Juliana Kaori ^[1] ; Carvalho, Andre Lopes ^[2] ; Vettore, Andre Luiz ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Paulo UNIFESP, Lab Mol Canc Biol, Dept Sci Biol, BR-04039032 Sao Paulo - Brazil

^[2] Barretos Canc Hosp, Dept Head & Neck Surg, BR-14784400 Barretos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ONCOLOGY REPORTS; v. 30, n. 1, p. 334-340, JUL 2013.
• Citações Web of Science: 7

Resumo

Mutations in JAK-STAT signaling pathway genes have been associated with the development of various hematological tumors, but have not been investigated in head and neck tumors, and the PIK3CA, BRAF and KRAS genes have been described in a few cases of head and neck squamous cell carcinoma (HNSCC). In the present study, we determined the mutation status in members of the MAPK, PI3K-AKT and JAK-STAT pathways in HNSCC. Mutations in the KRAS, BRAF, PIK3CA, JAK1 and JAK2 genes were evaluated in 94 HNSCCs by direct DNA sequencing analysis using cDNA synthesized from RNA extracted from patient tumor cells. All patients evaluated had wild-type KRAS, BRAF and PIK3CA genes. Furthermore, although some known polymorphisms have been found in JAK1 genes (rs45598436, rs17127063, rs2230587, rs3737139, rs2230588 and rs12129819) and JAK2 (rs10429491, rs2230723, rs2230724 and rs41316003), no mutation could be detected. Our data indicate that mutations in these kinase genes seem to be rare events in HNSCC. (AU)

• Processo FAPESP: 08/58460-9 - Avaliacao do perfil de metilacao como marcador molecular para o diagnostico precoce de recidivas em pacientes com tumores de cabeca e pescoco
• Beneficiário: Andre Luiz Vettore de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular