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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Higher microsatellite diversity in Plasmodium vivax than in sympatric Plasmodium falciparum populations in Pursat, Western Cambodia

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Autor(es):
Orjuela-Sanchez, Pamela [1, 2] ; Sa, Juliana M. [3] ; Brandi, Michelle C. C. [1] ; Rodrigues, Priscila T. [1] ; Bastos, Melissa S. [1] ; Amaratunga, Chanaki [3] ; Duong, Socheat [4] ; Fairhurst, Rick M. [3] ; Ferreira, Marcelo U. [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Sao Paulo - Brazil
[2] La Jolla Bioengn Inst, San Diego, CA - USA
[3] NIAID, Lab Malaria & Vector Res, NIH, Rockville, MD - USA
[4] Natl Ctr Parasitol Entomol & Malaria Control, Phnom Penh - Cambodia
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Experimental Parasitology; v. 134, n. 3, p. 318-326, JUL 2013.
Citações Web of Science: 34
Resumo

Previous microsatellite analyses of sympatric populations of Plasmodium vivax and Plasmodium falciparum in Brazil revealed higher diversity in the former species. However, it remains unclear whether regional species-specific differences in prevalence and transmission levels might account for these findings. Here, we examine sympatric populations of P. vivax (n = 87) and P. falciparum (n = 164) parasites from Pursat province, Western Cambodia, where both species are similarly prevalent. Using 10 genome-wide microsatellites for P. falciparum and 13 for P. vivax, we found that the P. vivax population was more diverse than the sympatric P. falciparum population (average virtual heterozygosity {[}H-E], 0.87 vs. 0.66, P = 0.003), with more multiple-clone infections (89.6% vs. 47.6%) and larger mean number of alleles per marker (16.2 vs. 11.1, P = 0.07). Both populations showed significant multi-locus linkage disequilibrium suggestive of a predominantly clonal mode of parasite reproduction. The higher microsatellite diversity found in P. vivax isolates, compared to sympatric P. falciparum isolates, does not necessarily result from local differences in transmission level and may reflect differences in population history between species or increased mutation rates in P. vivax. (C) 2013 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 07/51199-0 - Diversidade genetica, estrutura populacional e dinamica de transmissao de plasmodium vivax na amazonia rural brasileira.
Beneficiário:Marcelo Urbano Ferreira
Modalidade de apoio: Auxílio à Pesquisa - Regular