Chloroaluminium phthalocyanine polymeric nanoparticles as photosensitisers: Photophysical and physicochemical characterisation, release and phototoxicity in vitro

Autor(es)

de Paula, Carina Silva ^{[1, 2]} ; Tedesco, Antonio Claudio ^[3] ; Primo, Fernando Lucas ^[3] ; Carneiro Vilela, Jose Mario ^[4] ; Andrade, Margareth Spangler ^[4] ; Furtado Mosqueira, Vanessa Carla ^{[1, 2, 5]}

Afiliação do(s) autor(es)

^[1] Univ Fed Ouro Preto, Programa Posgrad Nanotecnol Farmaceut, BR-35400000 Ouro Preto, MG - Brazil

^[2] Univ Fed Ouro Preto, Programa Posgrad Ciencias Biol, BR-35400000 Ouro Preto, MG - Brazil

^[3] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ctr Nanotecnol & Engn Tecidos, Dept Quim, Lab Fotobiol & Fotomed, BR-14040901 Ribeirao Preto, SP - Brazil

^[4] Fundacao Ctr Tecnol Minas Gerais CETEC, BR-31170000 Belo Horizonte, MG - Brazil

^[5] Univ Fed Ouro Preto, Escola Farm, Ctr 171, Dept Farm, BR-35400000 Ouro Preto, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: European Journal of Pharmaceutical Sciences; v. 49, n. 3, p. 371-381, JUN 14 2013.
• Citações Web of Science: 32

Resumo

Nanoparticles of poly(D,L-lactide-co-glycolide), poly(D,L-lactide) and polyethylene glycol-block-poly(D,L-lactide) were developed to encapsulate chloroaluminium phthalocyanine (AlClPc), a new hydrophobic photosensitiser used in photodynamic therapy (PDT). The mean nanoparticle size varied from 115 to 274 nm, and the encapsulation efficiency ranged from 57% to 96% due to drug precipitation induced by different types of polymer. All nanoparticle formulations presented negative zeta potential values (-37 mV to -59 mV), explaining their colloidal stability. The characteristic photophysical parameters were analysed: the absorption spectrum profile, fluorescence quantum yield and transient absorbance decay, with similar values for free and nanoparticles of AlClPc. The time-resolved spectroscopy measurements for AlClPc triplet excited state lifetimes indicate that encapsulation in nanocapsules increases triplet lifetime, which is advantageous for PDT efficiency. A sustained release profile over 168 h was obtained using external sink method. An in vitro phototoxic effect higher than 80% was observed in human fibroblasts at low laser light doses (3 J/cm(2)) with 10 mu M of AlClPc. The AlClPc loaded within polymeric nanocapsules presented suitable physical stability, improved photophysical properties, sustained released profile and suitable activity in vitro to be considered a promising formulation for PDT. (c) 2013 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 07/55319-0 - Implementação e consolidação do serviço ambulatorial de tratamento de câncer de pele, junto a UNIFESP
• Beneficiário: Antonio Claudio Tedesco
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa em Políticas Públicas
• Processo FAPESP: 06/50562-1 - Avaliacao do uso de laser de baixa potencia e agentes fotossensibilizadores associados a sistemas de liberacao nanoestruturados no tratamento de feridas e/ou outras doencas cutaneas
• Beneficiário: Fernando Lucas Primo
• Modalidade de apoio: Bolsas no Brasil - Doutorado