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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Chloroaluminium phthalocyanine polymeric nanoparticles as photosensitisers: Photophysical and physicochemical characterisation, release and phototoxicity in vitro

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Author(s):
de Paula, Carina Silva [1, 2] ; Tedesco, Antonio Claudio [3] ; Primo, Fernando Lucas [3] ; Carneiro Vilela, Jose Mario [4] ; Andrade, Margareth Spangler [4] ; Furtado Mosqueira, Vanessa Carla [1, 2, 5]
Total Authors: 6
Affiliation:
[1] Univ Fed Ouro Preto, Programa Posgrad Nanotecnol Farmaceut, BR-35400000 Ouro Preto, MG - Brazil
[2] Univ Fed Ouro Preto, Programa Posgrad Ciencias Biol, BR-35400000 Ouro Preto, MG - Brazil
[3] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ctr Nanotecnol & Engn Tecidos, Dept Quim, Lab Fotobiol & Fotomed, BR-14040901 Ribeirao Preto, SP - Brazil
[4] Fundacao Ctr Tecnol Minas Gerais CETEC, BR-31170000 Belo Horizonte, MG - Brazil
[5] Univ Fed Ouro Preto, Escola Farm, Ctr 171, Dept Farm, BR-35400000 Ouro Preto, MG - Brazil
Total Affiliations: 5
Document type: Journal article
Source: European Journal of Pharmaceutical Sciences; v. 49, n. 3, p. 371-381, JUN 14 2013.
Web of Science Citations: 32
Abstract

Nanoparticles of poly(D,L-lactide-co-glycolide), poly(D,L-lactide) and polyethylene glycol-block-poly(D,L-lactide) were developed to encapsulate chloroaluminium phthalocyanine (AlClPc), a new hydrophobic photosensitiser used in photodynamic therapy (PDT). The mean nanoparticle size varied from 115 to 274 nm, and the encapsulation efficiency ranged from 57% to 96% due to drug precipitation induced by different types of polymer. All nanoparticle formulations presented negative zeta potential values (-37 mV to -59 mV), explaining their colloidal stability. The characteristic photophysical parameters were analysed: the absorption spectrum profile, fluorescence quantum yield and transient absorbance decay, with similar values for free and nanoparticles of AlClPc. The time-resolved spectroscopy measurements for AlClPc triplet excited state lifetimes indicate that encapsulation in nanocapsules increases triplet lifetime, which is advantageous for PDT efficiency. A sustained release profile over 168 h was obtained using external sink method. An in vitro phototoxic effect higher than 80% was observed in human fibroblasts at low laser light doses (3 J/cm(2)) with 10 mu M of AlClPc. The AlClPc loaded within polymeric nanocapsules presented suitable physical stability, improved photophysical properties, sustained released profile and suitable activity in vitro to be considered a promising formulation for PDT. (c) 2013 Elsevier B.V. All rights reserved. (AU)