Raman microspectroscopy and chemometrics for the characterization of polymer-based microcarrier systems of active/photoactive compounds

• Grant number: 16/18788-1
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2017
• End date: July 31, 2019
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Engineering
• Principal Investigator: Milena Martelli Tosi
• Grantee: Milena Martelli Tosi
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Associated researchers: Antonio Claudio Tedesco ; Odilio Benedito Garrido de Assis

Abstract

Nowadays, biocompatible polymers have been widely studied as carriers of active/photoactive compounds, such as essential vitamins and phthalocyanines (FC). Vitamins are widely added to food products and dietary supplements in order to enhance health benefits, while FC are photosensitizing agents employed in the photodynamic therapy. FC are activated by visible radiation in order to promote apoptosis or necrosis of tumor cells by different photophysical and photobiological events. Despite their different applications, biopolymers have been studied as carrier systems in order to achieve common objectives: to maintain stability and control of active release. However, few studies have explored the microspectroscopic characterization of these materials. Thus, the main objectives of this study are (i) to obtain biopolymeric carrier systems (chitosan micro/nanoparticles-MQ and/or cellulose micro/nanofibers-MC) as active compound carriers (folic acid or vitamin B6, cholecalciferol or vitamin D3 and FC), and (ii) the Raman microspectroscopic study in order to identify and map the regions of interaction between the components, determining heterogeneities and conformation changes between the substances. By means of chemometrics, the spectral data will be correlated to material properties. In this study, we have chosen MQ and MC due to their functional differences: MQ are essentially cationic in acid aqueous solution, and MC are essentially anionic. These features should change significantly the biological behavior of the photoactives. The relevance of this study is based on the importance of better understanding the material interactions for improving technological applications. Moreover, the approval of this project will allow the implementation of a new line of research in the department. (AU)

Scientific publications

(16)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TESSARO, LARISSA; LUCIANO, CARLA GIOVANA; BITTANTE, ANA MONICA QUINTA BARBOSA; LOURENCO, RODRIGO VINICIUS; MARTELLI-TOSI, MILENA; SOBRAL, PAULO JOSE DO AMARAL. Gelatin and/or chitosan-based films activated with ``Pitanga{''} (Eugenia uniflora L.) leaf hydroethanolic extract encapsulated in double emulsion. FOOD HYDROCOLLOIDS, v. 113, APR 2021. (13/07914-8, 16/18788-1)

ESPOSTO, BRUNO STEFANI; JAUREGI, PAULA; TAPIA-BLACIDO, DELIA R.; MARTELLI-TOSI, MILENA. Liposomes vs. chitosomes: Encapsulating food bioactives. TRENDS IN FOOD SCIENCE & TECHNOLOGY, v. 108, p. 40-48, FEB 2021. (19/22502-4, 19/23171-1, 19/05511-0, 16/18788-1)

DA SILVA, NATALIA CRISTINA; ASSIS, ODILIO BENEDITO GARRIDO; SARTORI, ALAN GIOVANINI DE OLIVEIRA; DE ALENCAR, SEVERINO MATIAS; MARTELLI-TOSI, MILENA. Chitosan suspension as extractor and encapsulating agent of phenolics from acerola by-product. Food Research International, v. 161, p. 8-pg., 2022-09-05. (16/18788-1, 19/23171-1)

OKAMOTO-SCHALCH, NADIA OBROWNICK; BOTELHO PINHO, SABRINA GUARDA; DE BARROS-ALEXANDRINO, TAIS TEO; DACANAL, GUSTAVO CESAR; GARRIDO ASSIS, ODILIO BENEDITO; MARTELLI-TOSI, MILENA. Production and characterization of chitosan-TPP/cellulose nanocrystal system for encapsulation: a case study using folic acid as active compound. Cellulose, v. 27, n. 10, p. 5855-5869, JUL 2020. (16/18788-1)

TESSARO, LARISSA; LOURENCO, RODRIGO VINICIUS; MARTELLI-TOSI, MILENA; SOBRAL, PAULO JOSE DO AMARAL. Gelatin/chitosan based films loaded with nanocellulose from soybean straw and activated with ``Pitanga{''} (Eugenia uniflora L.) leaf hydroethanolic extract in W/O/W emulsion. International Journal of Biological Macromolecules, v. 186, p. 328-340, SEP 1 2021. (16/18788-1, 13/07914-8)

TESSARO, LARISSA; LUCIANO, CARLA GIOVANA; MARTINS, MARIA FERNANDA LIBORIO; RAMOS, ANA PAULA; MARTELLI-TOSI, MILENA; DO AMARAL SOBRAL, PAULO JOSE. Stable and bioactive W/O/W emulsion loaded with ``Pitanga{''} (Eugenia uniflora L.) leaf hydroethanolic extract. JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY, JUN 2021. (13/07914-8, 16/18788-1)

VIEIRA RUBIO, FERNANDA THAIS; ISIDORO HAMINIUK, CHARLES WINDSON; DOS SANTOS, MAYARA MARTINS; THOMAZINI, MARCELO; FREITAS MORAES, IZABEL CRISTINA; MARTELLI-TOSI, MILENA; FAVARO-TRINDADE, CARMEN SILVIA. Development of natural pigments microencapsulated in waste yeast Saccharomyces cerevisiae using spray drying technology and their application in yogurt. FOOD & FUNCTION, v. 12, n. 19, JUL 2021. (18/12645-0, 16/18788-1)

ALINE SAYURI LIMA IIDA; KARINA NOVAIS LUZ; TAÍS TÉO BARROS-ALEXANDRINO; CARMEN SÍLVIA FÁVARO-TRINDADE; SAMANTHA CRISTINA DE PINHO; ODÍLIO BENEDITO GARRIDO ASSIS; MILENA MARTELLI-TOSI. Investigation of TPP-Chitosomes particles structure and stability as encapsulating agent of cholecalciferol. POLIMEROS-CIENCIA E TECNOLOGIA, v. 29, n. 4, 2020-04-06. (16/18788-1)

LARISSA TESSARO; MILENA MARTELLI-TOSI; PAULO JOSÉ DO AMARAL SOBRAL. Development of W/O emulsion for encapsulation of “Pitanga” (Eugenia uniflora L.) leaf hydroethanolic extract: droplet size, physical stability and rheology. FOOD SCIENCE AND TECHNOLOGY, n. ahead, p. -, 2021. (16/18788-1, 13/07914-8)

LARISSA TESSARO; MILENA MARTELLI-TOSI; PAULO JOSÉ DO AMARAL SOBRAL. Development of W/O emulsion for encapsulation of “Pitanga” (Eugenia uniflora L.) leaf hydroethanolic extract: droplet size, physical stability and rheology. FOOD SCIENCE AND TECHNOLOGY, v. 42, 2021-06-11. (13/07914-8, 16/18788-1)

DA SILVA, NATALIA CRISTINA; DE BARROS-ALEXANDRINO, TAIS TEO; GARRIDO ASSIS, ODILIO BENEDITO; MARTELLI-TOSI, MILENA. Extraction of phenolic compounds from acerola by-products using chitosan solution, encapsulation and application in extending the shelf-life of guava. Food Chemistry, v. 354, AUG 30 2021. (16/18788-1, 19/23171-1)

ESPOSTO, BRUNO STEFANI; BOTELHO PINHO, SABRINA GUARDA; THOMAZINI, MARCELO; RAMOS, ANA PAULA; TAPIA-BLACIDO, DELIA RITA; MARTELLI-TOSI, MILENA. TPP-chitosomes as potential encapsulation system to protect carotenoid-rich extract obtained from carrot by-product: A comparison with liposomes and chitosomes. Food Chemistry, v. 397, p. 8-pg., 2022-08-06. (19/22502-4, 19/05511-0, 16/18788-1, 19/23171-1)

VIEIRA RUBIO, FERNANDA THAIS; ISIDORO HAMINIUK, CHARLES WINDSON; DE FREITAS SANTOS, PRISCILA DAYANE; MARTELLI-TOSI, MILENA; THOMAZINI, MARCELO; DE CARVALHO BALIEIRO, JULIO CESAR; FIGUEIREDO MAKIMORI, GUSTAVO YASUO; FAVARO-TRINDADE, CARMEN SILVIA. Investigation on brewer's spent yeast as a bio-vehicle for encapsulation of natural colorants from pumpkin (Cucurbita moschata) peels. FOOD & FUNCTION, v. 13, n. 19, p. 14-pg., 2022-08-22. (16/18788-1)

SILVA, NATALIA C.; ESPOSTO, BRUNO S.; MANIGLIA, BIANCA C.; TAPIA-BLACIDO, DELIA R.; MARTELLI-TOSI, MILENA. Using Experimental Design and Response Surface Methodology to Optimize Nanocellulose Production from Two Types of Pretreated Soybean Straw. MACROMOLECULAR CHEMISTRY AND PHYSICS, v. 223, n. 18, p. 9-pg., 2022-08-09. (16/18788-1)

TESSARO, LARISSA; MARTELLI-TOSI, MILENA; DO AMARAL SOBRAL, PAULO JOSE. Development of W/O emulsion for encapsulation of "Pitanga" (Eugenia uniflora L.) leaf hydroethanolic extract: droplet size, physical stability and rheology. FOOD SCIENCE AND TECHNOLOGY, v. 42, p. 8-pg., 2022-01-01. (16/18788-1, 13/07914-8)

TESSARO, LARISSA; LUCIANO, CARLA GIOVANA; BITTANTE, ANA MONICA QUINTA BARBOSA; LOURENCO, RODRIGO VINICIUS; MARTELLI-TOSI, MILENA; SOBRAL, PAULO JOSE DO AMARAL. Gelatin and/or chitosan-based films activated with "Pitanga" (Eugenia uniflora L.) leaf hydroethanolic extract encapsulated in double emulsion. FOOD HYDROCOLLOIDS, v. 113, p. 12-pg., 2021-04-01. (13/07914-8, 16/18788-1)