Study of the Mechanisms of Action in Photodynamic Therapy: From Photosensitizer to Practical Application

• Grant number: 19/13569-8
• Support type: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): September 01, 2019
• Effective date (End): March 31, 2022
• Field of knowledge: Physical Sciences and Mathematics - Physics
• Principal researcher: Vanderlei Salvador Bagnato
• Grantee: Lucas Danilo Dias
• Home Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 13/07276-1 - CEPOF - Optics and Photonic Research Center, AP.CEPID

Abstract

Photodynamic therapy (PDT) is an alternative, selective and non-toxic technique that is applied to destroy cancer cells as well as pathogenic microorganisms, by light activation of a photosensitizer molecule. Its operation requires the conjunction of three fundamentals components: ideal photosensitizer, appropriate wavelength light source and oxygen. This project intends to carry out a systematic in vitro study regarding the ptimization/selection of photosensitizer structure/light source, to infer about the relationship between the mechanism of action of photodynamic therapy and the cellular location of the photosensitizer.Functionalization and characterization methods (UV-Vis, Infra-red and Raman spectroscopy) of solid supports / biomedical devices with optimal photosensitizers for the development of materials with biomedical application will be evaluated. Among them, we highlight solid biomaterials bearing immobilized photosensitizers capable of possessing antimicrobial properties by photodynamic action. In vitro studies will be carried out in strains of opportunistic bacteria causing skin and upper respiratory tract infections, such as Staphylococcus aureus, Eccherichia coli, Pseudomonas aeruginosa and Streptococcus pyogenes, and in addition, all cytotoxicity assays in human fibroblast cells will be performed. The most promising material(s) will be applied for in vivo (animal models) evaluation.

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DIAS, LUCAS D.; RODRIGUES, FABIO M. S.; CALVETE, MARIO J. F.; CARABINEIRO, SONIA A. C.; SCHERER, MARISA D.; CAIRES, ANDERSON R. L.; BUIJNSTERS, JOSEPHUS G.; FIGUEIREDO, JOSE L.; BAGNATO, VANDERLEI S.; PEREIRA, MARIETTE M. Porphyrin-Nanodiamond Hybrid Materials-Active, Stable and Reusable Cyclohexene Oxidation Catalysts. CATALYSTS, v. 10, n. 12 DEC 2020. Web of Science Citations: 0.

DIAS, LUCAS D.; MFOUO-TYNGA, IVAN S. Learning from Nature: Bioinspired Chlorin-Based Photosensitizers Immobilized on Carbon Materials for Combined Photodynamic and Photothermal Therapy. BIOMIMETICS, v. 5, n. 4 DEC 2020. Web of Science Citations: 0.

DIAS, LUCAS D.; BLANCO, KATE C.; MFOUO-TYNGA, IVAN S.; INADA, NATALIA M.; BAGNATO, VANDERLEI S. Curcumin as a photosensitizer: From molecular structure to recent advances in antimicrobial photodynamic therapy. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY C-PHOTOCHEMISTRY REVIEWS, v. 45, DEC 2020. Web of Science Citations: 0.

ZANGIROLAMI, AMANDA C.; DIAS, LUCAS D.; BLANCO, KATE C.; VINAGREIRO, CAROLINA S.; INADA, NATALIA M.; ARNAUT, LUIS G.; PEREIRA, MARIETTE M.; BAGNATO, VANDERLEI S. Avoiding ventilator-associated pneumonia: Curcumin-functionalized endotracheal tube and photodynamic action. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v. 117, n. 37, p. 22967-22973, SEP 15 2020. Web of Science Citations: 0.

SOARES, JENNIFER MACHADO; INADA, NATALIA MAYUMI; BAGNATO, VANDERLEI SALVADOR; BLANCO, KATE CRISTINA. Evolution of surviving Streptoccocus pyogenes from pharyngotonsillitis patients submit to multiple cycles of antimicrobial photodynamic therapy. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 210, SEP 2020. Web of Science Citations: 0.

DIAS, LUCAS D.; BAGNATO, VANDERLEI S. An update on clinical photodynamic therapy for fighting respiratory tract infections: a promising tool against COVID-19 and its co-infections. LASER PHYSICS LETTERS, v. 17, n. 8 AUG 2020. Web of Science Citations: 0.

BORGES, I. D.; DANIELLI, V, J. A.; SILVA, V. E. G.; SALLUM, L. O.; DE QUEIROZ, J. E.; DIAS, L. D.; IERMAK, I.; AQUINO, G. L. B.; CAMARGO, A. J.; VALVERDE, C.; OSORIO, F. A. P.; BASEIA, B.; NAPOLITANO, H. B. Synthesis and structural studies on (E)-3-(2,6-difluorophenyl)-1-(4-fluorophenyl)prop-2-en-1-one: a promising nonlinear optical material. RSC ADVANCES, v. 10, n. 38, p. 22542-22555, JUN 14 2020. Web of Science Citations: 0.

GONZALEZ, ANDREIA C. S.; DAMAS, LILIANA; AROSO, RAFAEL T.; TOME, VANESSA A.; DIAS, LUCAS D.; PINA, JOAO; CARRILHO, RUI M. B.; PEREIRA, MARLETTE M. Monoterpene-based metallophthalocyanines: Sustainable synthetic approaches and photophysical studies. JOURNAL OF PORPHYRINS AND PHTHALOCYANINES, v. 24, n. 5-7, p. 947-958, MAY-JUL 2020. Web of Science Citations: 0.