Photodynamic therapy (PDT) is an alternative, selective and non-toxic technique that is applied to destroy cancer cells as well as pathogenic microorganisms, by light activation of a photosensitizer molecule. Its operation requires the conjunction of three fundamentals components: ideal photosensitizer, appropriate wavelength light source and oxygen. This project intends to carry out a systematic in vitro study regarding the ptimization/selection of photosensitizer structure/light source, to infer about the relationship between the mechanism of action of photodynamic therapy and the cellular location of the photosensitizer.Functionalization and characterization methods (UV-Vis, Infra-red and Raman spectroscopy) of solid supports / biomedical devices with optimal photosensitizers for the development of materials with biomedical application will be evaluated. Among them, we highlight solid biomaterials bearing immobilized photosensitizers capable of possessing antimicrobial properties by photodynamic action. In vitro studies will be carried out in strains of opportunistic bacteria causing skin and upper respiratory tract infections, such as Staphylococcus aureus, Eccherichia coli, Pseudomonas aeruginosa and Streptococcus pyogenes, and in addition, all cytotoxicity assays in human fibroblast cells will be performed. The most promising material(s) will be applied for in vivo (animal models) evaluation.
News published in Agência FAPESP Newsletter about the scholarship: