Coenzyme Q10 protects Pc12 cells from cisplatin-induced DNA damage and neurotoxicity

Autor(es)

Machado, Carla da Silva ^[1] ; Mendonca, Leonardo Meneghin ^[1] ; Venancio, Vinicius de Paula ^[1] ; Pires Bianchi, Maria Lourdes ^[1] ; Greggi Antunes, Lusania Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Clin Toxicol & Bromatol Anal, Sch Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NeuroToxicology; v. 36, p. 10-16, MAY 2013.
• Citações Web of Science: 10

Resumo

The purpose of this study was to investigate the neuroprotective effect of a water-soluble formulation of coenzyme Q10 (WS-CoQ10) in PC12 cells exposed to cisplatin, a chemotherapeutic agent with a dose-limiting factor due to neurotoxicity. In the cytokinesis-block micronucleus cytome assay (CBMN Cyt), WS-CoQ10 (at concentrations of 0.1, 0.5 and 1.0 mu g mL(-1)) protected PC12 cells from cisplatin-induced DNA damage (0.1 mu g mL(-1)), reducing the frequency of micronuclei (MNi) and nuclear buds (NBUDs). WS-CoQ10 did not alter the mRNA expression levels of Tp53 (at a concentration of 1.0 mu g mL(-1)) and exhibited neuroprotective activity by stimulating cisplatin-inhibited neurite outgrowth in nerve growth factor (NGF)-differentiated PC12 cells (at a concentration of 0.1 mu g mL(-1)). In conclusion, WS-CoQ10 protected the PC12 cells from cisplatin-induced DNA damage and neurotoxicity. Moreover, the neuroprotective effects of WS-CoQ10 suggest a possible application in chemotherapeutic protocols. (c) 2013 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 08/53947-7 - Neurotoxicidade induzida pelo quimioterápico cisplatina: possíveis efeitos citoprotetores dos antioxidantes da dieta curcumina e coenzima Q10
• Beneficiário: Lusânia Maria Greggi Antunes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/11061-5 - Possíveis efeitos citoprotetores do antioxidante da dieta Coenzima Q10 em modelo de células neuronais
• Beneficiário: Carla da Silva Machado
• Modalidade de apoio: Bolsas no Brasil - Mestrado