Coenzyme Q10 protects Pc12 cells from cisplatin-induced DNA damage and neurotoxicity

Author(s)

Machado, Carla da Silva ^[1] ; Mendonca, Leonardo Meneghin ^[1] ; Venancio, Vinicius de Paula ^[1] ; Pires Bianchi, Maria Lourdes ^[1] ; Greggi Antunes, Lusania Maria ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Clin Toxicol & Bromatol Anal, Sch Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: NeuroToxicology; v. 36, p. 10-16, MAY 2013.
• Web of Science Citations: 10

Abstract

The purpose of this study was to investigate the neuroprotective effect of a water-soluble formulation of coenzyme Q10 (WS-CoQ10) in PC12 cells exposed to cisplatin, a chemotherapeutic agent with a dose-limiting factor due to neurotoxicity. In the cytokinesis-block micronucleus cytome assay (CBMN Cyt), WS-CoQ10 (at concentrations of 0.1, 0.5 and 1.0 mu g mL(-1)) protected PC12 cells from cisplatin-induced DNA damage (0.1 mu g mL(-1)), reducing the frequency of micronuclei (MNi) and nuclear buds (NBUDs). WS-CoQ10 did not alter the mRNA expression levels of Tp53 (at a concentration of 1.0 mu g mL(-1)) and exhibited neuroprotective activity by stimulating cisplatin-inhibited neurite outgrowth in nerve growth factor (NGF)-differentiated PC12 cells (at a concentration of 0.1 mu g mL(-1)). In conclusion, WS-CoQ10 protected the PC12 cells from cisplatin-induced DNA damage and neurotoxicity. Moreover, the neuroprotective effects of WS-CoQ10 suggest a possible application in chemotherapeutic protocols. (c) 2013 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 09/11061-5 - Possible cytoprotective effects of the dietary antioxidant coenzyme Q10 in a neuronal cell model
• Grantee: Carla da Silva Machado
• Support Opportunities: Scholarships in Brazil - Master