Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Coenzyme Q10 protects Pc12 cells from cisplatin-induced DNA damage and neurotoxicity

Full text
Machado, Carla da Silva [1] ; Mendonca, Leonardo Meneghin [1] ; Venancio, Vinicius de Paula [1] ; Pires Bianchi, Maria Lourdes [1] ; Greggi Antunes, Lusania Maria [1]
Total Authors: 5
[1] Univ Sao Paulo, Dept Clin Toxicol & Bromatol Anal, Sch Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: NeuroToxicology; v. 36, p. 10-16, MAY 2013.
Web of Science Citations: 10

The purpose of this study was to investigate the neuroprotective effect of a water-soluble formulation of coenzyme Q10 (WS-CoQ10) in PC12 cells exposed to cisplatin, a chemotherapeutic agent with a dose-limiting factor due to neurotoxicity. In the cytokinesis-block micronucleus cytome assay (CBMN Cyt), WS-CoQ10 (at concentrations of 0.1, 0.5 and 1.0 mu g mL(-1)) protected PC12 cells from cisplatin-induced DNA damage (0.1 mu g mL(-1)), reducing the frequency of micronuclei (MNi) and nuclear buds (NBUDs). WS-CoQ10 did not alter the mRNA expression levels of Tp53 (at a concentration of 1.0 mu g mL(-1)) and exhibited neuroprotective activity by stimulating cisplatin-inhibited neurite outgrowth in nerve growth factor (NGF)-differentiated PC12 cells (at a concentration of 0.1 mu g mL(-1)). In conclusion, WS-CoQ10 protected the PC12 cells from cisplatin-induced DNA damage and neurotoxicity. Moreover, the neuroprotective effects of WS-CoQ10 suggest a possible application in chemotherapeutic protocols. (c) 2013 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 09/11061-5 - Possible cytoprotective effects of the dietary antioxidant coenzyme Q10 in a neuronal cell model
Grantee:Carla da Silva Machado
Support type: Scholarships in Brazil - Master