Preventive effect of Ilex paraguariensis preparations (chimarrão, terere and mate tea) against damages caused by chronic ethanol consumption: comparative analysis of preparation technology, chemical profile and evaluation of therapeutic effect and safety

• Grant number: 17/03598-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2017
• End date: April 30, 2021
• Field of knowledge: Biological Sciences - Pharmacology - Toxicology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Marcos José Salvador
• Grantee: Mariana Mesquita Fonseca
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Alcoholism is defined by the 10th edition of the International Classification of Diseases (ICD-10) of the World Health Organization (WHO), as a set of behavioral, cognitive and physiological phenomena that develop after repeated use of alcohol. Psychiatric illness. The WHO estimates that there are 140 million people worldwide with alcoholism and in Brazil, the average consumption is superior to global, and it is documented in the literature that 53% of the adult population admits to consume alcoholic beverages. In this sense researches should be encouraged both to better understand the biological mechanisms of alcoholism as well as to search for therapeutic resources and with a view to detoxification. Ilex paraguariensis is a native plant native to South America of natural occurrence in Brazil, used in folk medicine and raw material for widely consumed beverages such as mate, tea and terrarium. It presents as major constituents phenolic substances, saponins and methylxanthines. However, it is known that the composition of its extract can be influenced by processes such as the drying of the plant (chimarrão) and roasting (tea maté), as well as by the procedure of preparation of the beverage (infusion time and water temperature) . Known for its antioxidant activity, recently effects on inhibition of glycation, hypocholesterolemic action, activities related to cardiovascular health, aid in the DNA repair system and neuroprotective effects, were also attributed to the extracts of this plant. In addition, there is a study demonstrating that extract from the commercial toast herb incorporated into the diet had a protective effect on some liver damage caused by the consumption of ethanol in rats. Thus, this doctoral work has as general objective to investigate the preventive effect of preparations with I. paraguariensis (chimarrão, terere and maté tea) against the damages caused by the chronic consumption of ethanol, including comparative analysis of preparation technology, chemical profile and Evaluation of therapeutic effect and safety. For this purpose, a comparative phytochemical study will be carried out between the aqueous extracts of I. paraguariensis obtained by the preparation technique of chimarrão, terere and maté tea, analyzing the chemical composition of each preparation (CG-MS, HPLC-UV / DAD-ESI / MS), in addition to the evaluation of antioxidant and anti-inflammatory activity in vitro in screening research. From the most promising extract in terms of antioxidant and anti-inflammatory activity, its cytotoxic and genotoxic effects will be investigated in vitro in cell cultures (NIH-3T3, AML-12, PC12) and in vivo with the use of Animal model (Wistar rats), in order to evaluate its preventive effect against the damages caused by the chronic consumption of ethanol. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA, CRISTHIAN SANTOS; ALVAREZ, CARLOS JOSE; PEREIRA CABRAL, MARCIA REGINA; SARRAGIOTTO, MARIA HELENA; SALVADOR, MARCOS JOSE; ALVES STEFANELLO, MARIA ELIDA. Three new diterpenoids from the leaves of Salvia lachnostachys. NATURAL PRODUCT RESEARCH, DEC 2021. (17/03598-5, 12/09101-1, 06/06079-4, 15/03726-8)

MESQUITA, M.; SANTOS, E.; KASSUYA, C. A.; SALVADOR, M. J.. Chimarra tilde o, terere and mate-tea in legitimate technology modes of preparation and consume: A comparative study of chemical composition, antioxidant, anti-inflammatory and anti-anxiety properties of the mostly consumed beverages of Ilex paraguariensis St. Hil.. Journal of Ethnopharmacology, v. 279, OCT 28 2021. (17/03598-5, 06/06079-4, 12/09101-1, 15/03726-8)

SILVA, ADSON S.; AMORIM, MAGALI S.; FONSECA, MARIANA M.; SALVADOR, MARCOS J.; DE SA, EDUARDO L.; STEFANELLO, MARIA ELIDA A.. A New Cytotoxic Naphthoquinone and Other Chemical Constituents of Sinningia reitzii. Journal of the Brazilian Chemical Society, v. 30, n. 10, SI, p. 2060-2065, OCT 2019. (15/03726-8, 17/03598-5)

VERDAN, MARIA HELENA; WINIEWSKI, VANESSA; SCHARF, DILAMARA R.; SALVADOR, MARCOS JOSE; DE SA, EDUARDO L.; STEFANELLO, MARIA ELIDA A.. imeric naphthochromenes and other constituents from Sinningia allagophylla (Gesneriaceae. PHYTOCHEMISTRY LETTERS, v. 42, p. 104-108, APR 2021. (06/06079-4, 12/09101-1, 15/03726-8, 17/03598-5)

HERNANDEZ-TASCO, ALVARO JOSE; TRONCHINI, RAFAELA APARECIDA; APAZA-CASTILLO, GLADYS ANGELICA; HOSAKA, GUILHERME KENICHI; QUINONES, NATALY RUIZ; GOULART, MARCELA CRISTINA; FANTINATTI-GARBOGGINI, FABIANA; SALVADOR, MARCOS JOSE. Diversity of bacterial and fungal endophytic communities presents in the leaf blades of Sinningia magnifica, Sinningia schiffneri and Sinningia speciosa from different cladus of Gesneriaceae family: A comparative analysis in three consecutive years. MICROBIOLOGICAL RESEARCH, v. 271, p. 10-pg., 2023-04-01. (21/09693-5, 15/03726-8, 17/03598-5, 06/06079-4, 12/09101-1)

TERCO LEITE, PATRICIA REGINA; LORENCONE, BETHANIA ROSA; TAFARELO MORENO, KARYNE GARCIA; LOPES, KATIANA SIMOES; MACEDO MARQUES, ALINE APARECIDA; FORTINI, CLARA SOLIGO; CALLOI PALOZI, RHANANY ALAN; DALMAGRO, MARIANA; LEITE KASSUYA, CANDIDA APARECIDA; DOS SANTOS, ARIANY CARVALHO; et al. The NO-cGMP-K (+) Channel Pathway Participates in Diuretic and Cardioprotective Effects of Blutaparon portulacoides in Spontaneously Hypertensive Rats. Planta Medica, v. 88, n. 13, p. 11-pg., 2022-03-17. (06/06079-4, 15/03726-8, 12/09101-1, 17/03598-5)