Efficacy of decaffeinated mate tea (Ilex paraguariensis) in the control of oxidative stress, obesity and bone damage in Wistar rat

• Grant number: 17/07628-6
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2018
• End date: April 30, 2020
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Paulo César Ciarlini
• Grantee: Paulo César Ciarlini
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• City of the host institution: Araçatuba

Abstract

Obesity is a chronic disease of high global incidence that affects humans and dogs. Cola-based refrigerant is an important component of the cafeteria diet associated with obesity and osteopathy in humans and rats. There is evidence that the consumption of mate tea (MT) contributes to minimize the undesirable effects of excess fat (chronic inflammation, oxidative stress and osteopathy). However, the anti-obesity, anti-inflammatory and anti-oxidative stress mechanisms of MT that prevent osteoporosis have not yet been well established. The therapeutic use of MT is contraindicated in humans and animals sensitive to caffeine. Thus, we propose to investigate the hypothesis that decaffeinated CM maintains its ability to minimize accumulation of body fat, bone damage associated with inflammation and systemic oxidative stress in rats submitted to excessive consumption of cola-based refrigerant. For this, a randomized armored study will be carried out, where 60 adult Wistar rats will be randomly fed for 90 days with and without cola-based refrigerant, with and without treatment with decaffeinated MT and non-decaffeinated MT. The control group will be compared to the other groups regarding their body composition (weight and Lee Index), plasma biochemical profile (hepatic transaminases, total protein, glucose, cholesterol, GGT, insulin, leptin, adiponectin, osteocalcin, triglycerides, total calcium, phosphorus and alkaline phosphatases), systemic oxidative stress (total antioxidant capacity (TAC) and total oxidant concentration (TOC)), plasma lipid peroxidation (TBARS) and plasma concentration of endogenous antioxidants (albumin, bilirubin and uric acid). To quantify the degree of tissue oxidative stress (hepatic, renal and bone) will be measured TBARS, the enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx). To evaluate the inflammatory response will be quantified the concentration of C-reactive protein, TNF±, IL-1, IL-6 and IL-10 in plasma. Osteocalcin (OCN), osteoprotegerin (OPG), tartrate-resistant acid phosphatase (TRAP), parathyroid hormone (PTH) and vitamin D will be measured in bone tissue. In the bone tissue will be measured the molecular conductors of osteoclastogenesis (OPG / RANKL / RANK), density and mineral content. It is hoped that the results obtained will contribute to a better understanding of the action of MT as an antioxidant, in the control of obesity and osteoporosis. Confirmation that decaffeinated MT maintains the same therapeutic properties will enable its use in humans and animals sensitive to caffeine. (AU)