Production of 6-kestose by the filamentous fungus Gliocladium virens as affected by sucrose concentration

Autor(es)

Fialho, Mauricio Batista ^[1] ; Simoes, Kelly ^[1] ; Barros, Caroline de Almeida ^[1] ; Bom Pessoni, Rosemeire Aparecida ^[2] ; Braga, Marcia Regina ^[1] ; Leone Figueiredo-Ribeiro, Rita de Cassia ^[1]

Afiliação do(s) autor(es)

^[1] Inst Bot, Nucleo Pesquisa Fisiol & Bioquim, BR-04045972 Sao Paulo - Brazil

^[2] Univ Metodista Sao Paulo, Fac Saude, BR-09735460 Sao Bernardo Do Campo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MYCOSCIENCE; v. 54, n. 3, p. 198-205, MAY 2013.
• Citações Web of Science: 9

Resumo

The filamentous fungus Gliocladium virens is able to produce fructooligosaccharides (FOS), fructose-containing sugars, used as functional ingredients to improve nutritional and technological properties of foods. In this work we evaluated FOS production by G. virens when grown in a wide range of sucrose concentrations (10-400 g l(-1)). High sucrose concentrations increased both biomass and FOS production, including 6-kestose, a trisaccharide comprising beta (2 --> 6) linked fructosyl units, with enhanced stability and prebiotic activity when compared to the typical FOS beta (2 -> 1) linked. The highest 6-kestose yield (3 g l(-1)) was achieved in media containing 150 g l(-1) sucrose after 4-5 days of culture, production being 90% greater than in media containing 10, 30, or 50 g l(-1) sucrose. After 5 days, FOS production declined markedly, following complete sucrose depletion in the medium. Although most of the beta-fructofuranosidases preferentially catalyze sucrose hydrolysis, FOS production in G. virens grown in high sucrose concentration, might be attributed to a reverse hydrolysis by these enzymes. In conclusion, high sucrose concentrations increase growth of G. Wrens whilst 6-kestose accumulation in the medium seems to be controlled both by specific properties of beta-fructofuranosidases and on the sucrose concentration. (C) 2012 The Mycological Society of Japan. Published by Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 05/04139-7 - Carboidratos de plantas tropicais como moduladores de processos ecofisiológicos e indicadores de respostas a estresses ambientais
• Beneficiário: Marcia Regina Braga
• Modalidade de apoio: Auxílio à Pesquisa - Temático