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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Production of 6-kestose by the filamentous fungus Gliocladium virens as affected by sucrose concentration

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Author(s):
Fialho, Mauricio Batista [1] ; Simoes, Kelly [1] ; Barros, Caroline de Almeida [1] ; Bom Pessoni, Rosemeire Aparecida [2] ; Braga, Marcia Regina [1] ; Leone Figueiredo-Ribeiro, Rita de Cassia [1]
Total Authors: 6
Affiliation:
[1] Inst Bot, Nucleo Pesquisa Fisiol & Bioquim, BR-04045972 Sao Paulo - Brazil
[2] Univ Metodista Sao Paulo, Fac Saude, BR-09735460 Sao Bernardo Do Campo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: MYCOSCIENCE; v. 54, n. 3, p. 198-205, MAY 2013.
Web of Science Citations: 9
Abstract

The filamentous fungus Gliocladium virens is able to produce fructooligosaccharides (FOS), fructose-containing sugars, used as functional ingredients to improve nutritional and technological properties of foods. In this work we evaluated FOS production by G. virens when grown in a wide range of sucrose concentrations (10-400 g l(-1)). High sucrose concentrations increased both biomass and FOS production, including 6-kestose, a trisaccharide comprising beta (2 --> 6) linked fructosyl units, with enhanced stability and prebiotic activity when compared to the typical FOS beta (2 -> 1) linked. The highest 6-kestose yield (3 g l(-1)) was achieved in media containing 150 g l(-1) sucrose after 4-5 days of culture, production being 90% greater than in media containing 10, 30, or 50 g l(-1) sucrose. After 5 days, FOS production declined markedly, following complete sucrose depletion in the medium. Although most of the beta-fructofuranosidases preferentially catalyze sucrose hydrolysis, FOS production in G. virens grown in high sucrose concentration, might be attributed to a reverse hydrolysis by these enzymes. In conclusion, high sucrose concentrations increase growth of G. Wrens whilst 6-kestose accumulation in the medium seems to be controlled both by specific properties of beta-fructofuranosidases and on the sucrose concentration. (C) 2012 The Mycological Society of Japan. Published by Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 05/04139-7 - Carbohydrates of tropical species as modulators of ecophysiological processes and as environmental stress response markers
Grantee:Marcia Regina Braga
Support Opportunities: Research Projects - Thematic Grants