| Texto completo | |
| Autor(es): |
da Cruz-Hoefling, Maria Alice
[1]
;
Raposo, Catarina
[1]
;
Verinaud, Liana
[2]
;
Zago, Gabriela Mariotoni
[1, 3]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Campinas, Inst Biol, Dept Histol & Embryol, BR-13083970 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Inst Biol, Dept Microbiol & Immunol, BR-13083970 Campinas, SP - Brazil
[3] Univ Estadual Campinas, Fac Med Sci, Dept Farmacol, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | NeuroToxicology; v. 30, n. 4, p. 636-646, July 2009. |
| Área do conhecimento: | Ciências Biológicas - Morfologia |
| Citações Web of Science: | 12 |
| Assunto(s): | Venenos de origem animal Animais peçonhentos Aranhas Citocinas Neurônios Astrócitos |
| Resumo | |
Phoneutria nigriventer spider venom (PNV) causes uneven BBB permeability throughout different cerebral regions. Little is known about cellular and molecular responses which course with the PNVinduced BBB opening. We investigate by immunohistochemistry (IHC) and Western blotting (WB), the GFAP, S100, IFN-g and TNF-a proteins expression in hippocampus and cerebellum after different timepoints from venom or saline intravenous injection. All proteins variably altered its expression temporally and regionally. WB showed increased GFAP content at 15-45 min followed by a shift below the control level which was less pronounced in hippocampus. IHC showed reactive gliosis during all the trial period. In cerebellum, GFAP was mostly immunodetected in astrocytes of the molecular layer (Bergmann glia), as was S100 protein. The maximum S100 immunolabeling was achieved at 5 h. IFN-g and TNF-a, expressed mostly by hippocampal neurons, increased along the trial period, suggesting a role in BBB permeability. In envenomed animals, closer contacts astrocyte-astrocyte, granule cells-granule cells and astrocytes-Purkinje cells were observed in cerebellum. Closer contacts between neurons-neurons-astrocytes-astrocytes were also seen in hippocampus. PNV contains serotonin, histamine, Ca2+ channels-blocking toxins, some of which affect glutamate release. The hypothesis that such substances plus the cytokines generated, could have a role in BBB permeability, and that calcium homeostasis loss and disturbance of glutamate release are associated with themarked GFAP/S100 reaction in Bergmann glia is discussed. The existence of a CNS mechanism of defense modulated differentially for fast synthesis and turnover of GFAP, S100, IFN-g and TNF-a proteins was evident... (AU) | |
| Processo FAPESP: | 07/50272-6 - Veneno e toxina de aranha phoneutria nigriventer: acao no sistema nervoso central. |
| Beneficiário: | Catarina Raposo Dias Carneiro |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 98/00341-0 - Estudo da acao nefrotoxica do veneno de bothrops moojeni "in vivi" em rim isolado e cultura de celulas renais. |
| Beneficiário: | Maria Alice da Cruz Hofling |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |