Use of electrospray tandem mass spectrometry for identification of microcystins during a cyanobacterial bloom event

Autor(es)

Frias, Humberto Vieira ; Mendes, Maria Anita ; Cardozo, Karina Helena Morais ; Carvalho, Valdemir Melechco ; Tomazela, Daniela ; Colepicolo, Pio ; Pinto, Ernani ^[7]

• Tipo de documento: Artigo Científico
• Fonte: Biochemical and Biophysical Research Communications; v. 344, n. 3, p. 741-746, June 2006.
• Área do conhecimento: Ciências da Saúde - Farmácia
• Assunto(s): Cianobactérias Microcistinas Reservatórios Represas Espectrometria de massas em Tandem

Resumo

Drastic environmental conditions such as elevated temperature, abrupt pH variation, low turbulence, and high nutrient inputs can enhance the development of toxic cyanobacterial blooms in lakes and reservoirs. This study describes the occurrence of four microcystin variants (MC) in a bloom in the eutrophic reservoir Billings, in São Paulo City. The bloom sample was collected in October 2003, and Microcystis were the main genus found. The MC were separated and purified by reverse phase high performance liquid chromatography (RP-HPLC). Their structures were elucidated by electrospray ionization tandem mass spectrometry (ESI-MS/MS) and four MC variants were determined: MC-RR, MC-LR, MC-YR, and MC-hRhR. MC-hRhR is described for the first time as a new variant of MC with two homoarginines at positions 2 and 4 in its structure. ESI-MS/MS analysis thus provides a powerful and convenient tool for the determination of variants of MC. These results represent an important contribution to the knowledge of the biochemistry of toxic cyanobacteria and their toxins, specifically in São Paulo State. (AU)

• Processo FAPESP: 04/14264-0 - Avaliacao dos possiveis mecanismos de acao das microcistinas sobre a funcao de neutrofilos humanos.
• Beneficiário: Ernani Pinto Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 03/06443-0 - Algas presentes em florações: isolamento e padronização de métodos analíticos para a quantificação das toxinas
• Beneficiário: Ernani Pinto Junior
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa em Políticas Públicas