Dietary phytosterol does not accumulate in the arterial wall and prevents atherosclerosis of LDLr-KO mice

Autor(es):Mostrar menos -

Bombo, Renata P. A. ^[1] ; Afonso, Milessa S. ^[1] ; Machado, Roberta M. ^[1] ; Ferrari Lavrador, Maria Silvia ^[1] ; Nunes, Valeria S. ^[1] ; Quintao, Eder R. ^[1] ; Koike, Marcia ^[2] ; Catanozi, Sergio ^[1] ; Lin, Chin Jia ^[3] ; Nakandakare, Edna R. ^[1] ; Lottenberg, Ana Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Sci, Lipids Lab, Div Endocrinol & Metab, BR-01246000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med Sci, Dept Med, Div Emergency Med, BR-01246000 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med Sci, Dept Pathol, Lab Mol Pathol, BR-01246000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ATHEROSCLEROSIS; v. 231, n. 2, p. 442-447, DEC 2013.
• Citações Web of Science: 13

Resumo

Scope: There have been conflicting reports on the usefulness of phytosterols (PS) in preventing atherosclerosis. We evaluated the effects of dietary PS supplementation in LDLr-KO male mice on the plasma and aorta sterol concentrations and on atherosclerotic lesion development. Methods and results: Mice were fed a high fat diet (40% of energy) supplemented with or without PS (2% w/w, n = 10). Plasma and arterial wall cholesterol and PS concentrations, lesion area, macrophage infiltration, and mRNA expression from LOX-1, CD36, ABCA1 and ABCG1 in peritoneal macrophages were measured. After 16 weeks, the plasma cholesterol concentration in PS mice was lower than that in the controls (p = 0.02) and in the arterial wall (p = 0.03). Plasma PS concentrations were higher in PS-fed animals than in controls (p < 0.0001); however, the arterial wall PS concentration did not differ between groups. The atherosclerotic lesion area in the PS group (n = 5) was smaller than that in controls (p = 0.0062) and the macrophage area (p = 0.0007). PS correlates negatively with arterial lipid content and macrophage (r = -0.76; p < 0.05). PS supplementation induced lower ABCG1 mRNA expression (p < 0.05). Conclusions: Despite inducing an increase in PS plasma concentration, PS supplementation is not associated with its accumulation in the arterial wall and prevents atherosclerotic lesion development. (C) 2013 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 11/50239-4 - Ação dos fitoesteróis sobre lesão aterosclerótica em camundongos com ablação gênica de receptor de LDL
• Beneficiário: Ana Maria Pita Lottenberg
• Modalidade de apoio: Auxílio à Pesquisa - Regular