Toxoplasma gondii in free-ranging wild small felids from Brazil: Molecular detection and genotypic characterization

Autor(es)

Canon-Franco, W. A. ^{[1, 2]} ; Araujo, F. A. P. ^[1] ; Lopez-Orozco, N. ^[3] ; Jardim, M. M. A. ^[4] ; Keid, L. B. ^[5] ; Dalla-Rosa, C. ^[6] ; Cabral, A. D. ^[7] ; Pena, H. F. J. ^[7] ; Gennari, S. M. ^[7]

Afiliação do(s) autor(es)

^[1] Univ Fed Rio Grande do Sul, Fac Vet, Dept Patol & Clin Vet, BR-91540000 Porto Alegre, RS - Brazil

^[2] Univ Caldas, Fac Ciencias Agr, Dept Salud Anim, Manizales - Colombia

^[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508000 Sao Paulo - Brazil

^[4] Fundacao Zoobot Rio Grande do Sul, Museu Ciencias Nat, BR-90690000 Porto Alegre, RS - Brazil

^[5] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, BR-13635900 Pirassununga, SP - Brazil

^[6] Univ Caxias Do Sul, Museu Ciencias Nat, BR-95070560 Caxias Do Sul, RS - Brazil

^[7] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, BR-05508270 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Veterinary Parasitology; v. 197, n. 3-4, p. 462-469, NOV 8 2013.
• Citações Web of Science: 14

Resumo

Brazil harbors the largest number of wild Neotropical felid species, with ten of the twelve species recorded in the American continent. Although these animals are considered to be definitive hosts for Toxoplasma gondii, there are few descriptions of the parasite in these species. Here, we performed a molecular detection of T. gondii by amplification of the marker ITS-1 from tissue samples obtained from 90 free-ranging wild small Neotropical felids from Rio Grande do Sul - Brazil. Of the sampled animals, 34.4% (n=31) were positive including the species Puma yagouaroundi - jaguarondi (9/22), Leopardus geoffroyi - Geoffroy's cat (6/22), Leopard us tigrinus - oncilla (8/28), Leopardus wiedii - margay (6/10), Leopardus pardalis - ocelot (1/1) and Leopard us colocolo - Pampas cat (1/7). Toxoplasma DNA was detected with a frequency of 14.6% (63/433) in primary samples of tongue (16/56), brain (8/43), skeletal muscle (15/83), heart (7/63), diaphragm (3/56), vitreous humor (2/44), eye muscle (6/44) and eyeball (6/44). Multilocus PCR-RFLP genotyping of eleven small Neotropical felids using the molecular markers SAG1, 5'3'SAG2, alt. SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico and CS3 allowed the partial characterization of eight genotypes. We fully characterized two new genotypes that have not been described previously in Brazil (Lw\#31Tn from L wiedii and Py\#21Sm from P. yagouaroundi) and one genotype Py\#56Br from P. yagouaroundi that has been described previously in isolates from cats, dogs and capybaras from Sao Paulo state. This study constitutes the first detection and genotypic characterization of T. gondii in free-ranging felids in Brazil, demonstrating the occurrence of the parasite in wild populations and suggesting its potential transmissibility to humans and other domestic and wild animals. (C) 2013 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 10/52308-0 - Detecção molecular de coccídios da família Sarcocystidae (subfamílias Sarcocystinae e Toxoplasmatinae) em amostras teciduais de felinos neotropicais coletadas em museus do estado do Rio Grande do Sul
• Beneficiário: Solange Maria Gennari
• Modalidade de apoio: Auxílio à Pesquisa - Regular