Differential pulse adsorptive stripping voltammetric determination of methotrexate using a functionalized carbon nanotubes-modified glassy carbon electrode

Autor(es)

Oliveira, Geiser G. ^[1] ; Janegitz, Bruno C. ^[2] ; Zucolotto, Valtencir ^[2] ; Fatibello-Filho, Orlando ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Chem, Ctr Sci & Technol, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Nanomed & Nanotoxicol Grp, Sao Carlos Inst Phys, BR-13566590 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CENTRAL EUROPEAN JOURNAL OF CHEMISTRY; v. 11, n. 11, p. 1837-1843, NOV 2013.
• Citações Web of Science: 15

Resumo

A glassy carbon electrode (GC) containing multiwalled functionalized carbon nanotubes (MWCNTs) immobilized within a dihexadecylhydrogenphosphate film (DHP) is proposed as a nanostructured platform for determination of methotrexate (MTX) concentration (a drug used in cancer treatment) using differential pulse adsorptive stripping voltammetry (DPAdSV). The voltammograms for a MTX solution using MWCNTs-DHP/GC electrode presented an oxidation peak potential at 0.98 V vs. Ag/AgCl (3.0 mol L-1 KCl) in a 0.1 mol L-1 sulphuric acid. The apparent heterogeneous electron transfer rate constant of 0.46 s(-1) was calculated. The recovery area of 2.62 x 10(-9) mol cm(2) was also obtained. Under the optimal experimental conditions, the analytical curve was linear in the MTX concentration range from 5.0 x 10(-8) to 5.0 x 10(-6) mol L-1, with a detection limit of 3.3 x 10(-8) mol L-1. The MWCNTs-DHP/GC electrode can be easily prepared and was applied for the determination of MTX in pharmaceutical formulations, with results similar to those obtained using a high-performance liquid chromatography comparative method. (AU)

• Processo FAPESP: 08/09893-0 - Desenvolvimento de biossensores utilizando nanotubos de carbono e nanopartículas
• Beneficiário: Bruno Campos Janegitz
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/02877-4 - Desenvolvimento de Novos Biossensores utilizando Materiais Nanoestruturados Ativos
• Beneficiário: Bruno Campos Janegitz
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado