Evaluation of Genotoxicity and Antigenotoxicity of Artepillin C in V79 Cells by the Comet and Micronucleus Assays

Autor(es)

de Oliveira, Pollyanna Francielli ^[1] ; de Souza Lima, Ildercilio Mota ^[1] ; Bentes Monteiro Neto, Moacir de Azevedo ^[1] ; Bastos, Jairo Kenupp ^[2] ; da Silva Filho, Ademar Alves ^[3] ; Tavares, Denise Crispim ^[1]

Afiliação do(s) autor(es)

^[1] Univ Franca, BR-14404611 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14049 Ribeirao Preto, SP - Brazil

^[3] Univ Fed Juiz de Fora, Dept Ciencias Farmaceut, Fac Farm, Juiz De Fora, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL; v. 65, n. 7, p. 1098-1103, OCT 1 2013.
• Citações Web of Science: 3

Resumo

Artepillin C (3,5-diprenyl-p-coumaric acid) is one of the major phenolic compounds found in Brazilian green propolis, as well as in its botanical source, Baccharis dracunculifolia DC (Asteraceae). The present study evaluated the possible genotoxic and protective activities of artepillin C, in vitro, using methyl methanesulfonate (MMS) as a positive control, by comet and micronucleus assays. The cultures of Chinese hamster lung fibroblasts (V79 cells) were treated with different concentrations of artepillin C (2.5, 5.0, 10.0, and 20 M). In antigenotoxicity assessment, the 3 concentrations of artepillin C (2.5, 5.0, and 10.0M) were associated with MMS (200M-comet assay and 400M-micronucleus assay). A statistically significant increase in the DNA damage and micronucleus frequencies was observed in the culture treated with the highest concentration of the artepillin C in comparison to the control group. All concentrations of artepillin C showed protective activity in relation to MMS-induced genotoxicity, which may be due to its antioxidant properties. (AU)

• Processo FAPESP: 09/05944-1 - Avaliação in vivo do potencial mutagênico/genotóxico e antimutagênico/antigenotóxico do Artepelin C
• Beneficiário: Ildercílio Mota de Souza Lima
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 07/07211-6 - Estudo da atividade mutagênica e/ou antimutagênica da Baccharis dracunculifolia e do Artepelin C em células de mamíferos
• Beneficiário: Denise Crispim Tavares Barbosa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/12019-2 - Avaliação in vivo e in vitro do potencial mutagênico/antimutagênico e genotóxico/antigenotóxico da baccharina, um importante composto químico da Baccharis dracunculifolia
• Beneficiário: Pollyanna Francielli de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Mestrado