Photodynamic inactivation of clinical isolates of Candida using Photodithazine (R)

Autor(es)

Dovigo, L. N. ^[1] ; Carmello, J. C. ^[2] ; Carvalho, M. T. ^[3] ; Mima, E. G. ^[2] ; Vergani, C. E. ^[2] ; Bagnato, V. S. ^[3] ; Pavarina, A. C. ^[2]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Araraquara Dent Sch, Dept Social Dent, Araraquara - Brazil

^[2] UNESP Univ Estadual Paulista, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, Araraquara - Brazil

^[3] Univ Sao Paulo, Phys Inst Sao Carlos, Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOFOULING; v. 29, n. 9, p. 1057-1067, OCT 1 2013.
• Citações Web of Science: 24

Resumo

This study evaluated the photodynamic inactivation (PDI) mediated by Photodithazine((R)) (PDZ) against 15 clinical isolates of Candida albicans, Candida glabrata and Candida tropicalis. Each isolate, in planktonic and biofilm form, was exposed to PDI by assessing a range of PDZ concentrations and light emitting diode fluences. Cell survival of the planktonic suspensions was determined by colony forming units (CFU ml(-1)). The antifungal effects of PDI against biofilms were evaluated by CFUml(-1) and metabolic assay. Data were analyzed by non-parametric tests (=0.05). Regardless of the species, PDI promoted a significant viability reduction of planktonic yeasts. The highest reduction in cell viability of the biofilms was equivalent to 0.9 log(10) (CFU ml(-1)) for C. albicans, while 1.4 and 1.5 log(10) reductions were obtained for C. tropicalis and C. glabrata, respectively. PDI reduced the metabolic activity of biofilms by 62.1, 76.0, and 76.9% for C. albicans, C. tropicalis, and C. glabrata, respectively. PDZ-mediated PDI promoted significant reduction in the viability of Candida isolates. (AU)

• Processo FAPESP: 08/00601-6 - Estudo da efetividade da Terapia Fotodinâmica Antimicrobiana na inativação de espécies de Candida e do dano celular causado nas células fúngicas. Avaliação in vitro e in vivo de dois fotossensibilizadores.
• Beneficiário: Lívia Nordi Dovigo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 98/14270-8 - Centro de Ciências Ópticas e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 08/03994-9 - Estudo da efetividade da terapia fotodinâmica antimicrobiana na inativação de espécies de Candida e do dano celular causado nas células fúngicas: avaliação in vitro e in vivo de dois fotossensibilizadores
• Beneficiário: Ana Cláudia Pavarina
• Modalidade de apoio: Auxílio à Pesquisa - Regular