Human respiratory syncytial virus N, P and M protein interactions in HEK-293T cells

Autor(es)

Oliveira, Andressa P. ^[1] ; Simabuco, Fernando M. ^{[1, 2]} ; Tamura, Rodrigo E. ^{[1, 3]} ; Guerrero, Manuel C. ^[4] ; Ribeiro, Paulo G. G. ^[1] ; Libermann, Towia A. ^[4] ; Zerbini, Luiz F. ^{[4, 5, 6]} ; Ventura, Armando M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Microbiol, Inst Ciencias Biomed, BR-05508900 Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Fac Ciencias Aplicadas, Sao Paulo - Brazil

^[3] Inst Canc Estado Sao Paulo Octavio Frias de Olive, Ctr Invest Translac Oncol CTO, Sao Paulo - Brazil

^[4] Harvard Univ, Sch Med, BIDMC Genom Ctr, Boston, MA - USA

^[5] Univ Cape Town, ICGEB, ZA-7925 Cape Town - South Africa

^[6] Univ Cape Town, Div Med Biochem, ZA-7925 Cape Town - South Africa

• Tipo de documento: Artigo Científico
• Fonte: VIRUS RESEARCH; v. 177, n. 1, p. 108-112, OCT 2013.
• Citações Web of Science: 17

Resumo

Characterization of Human Respiratory Syncytial Virus (HRSV) protein interactions with host cell components is crucial to devise antiviral strategies. Viral nucleoprotein, phosphoprotein and matrix protein genes were optimized for human codon usage and cloned into expression vectors. HEK-293T cells were transfected with these vectors, viral proteins were immunoprecipitated, and co-immunoprecipitated cellular proteins were identified through mass spectrometry. Cell proteins identified with higher confidence scores were probed in the immunoprecipitation using specific antibodies. The results indicate that nucleoprotein interacts with arginine methyl-transferase, methylosome protein and Hsp70. Phosphoprotein interacts with Hsp70 and tropomysin, and matrix with tropomysin and nucleophosmin. Additionally, we performed immunoprecipitation of these cellular proteins in cells infected with HRSV, followed by detection of co-immunoprecipitated viral proteins. The results indicate that these interactions also occur in the context of viral infection, and their potential contribution for a HRSV replication model is discussed. (C) 2013 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 09/17587-9 - Estudos de imunização e moleculares sobre o Vírus Respiratório Sincicial Humano
• Beneficiário: Armando Morais Ventura
• Modalidade de apoio: Auxílio à Pesquisa - Regular