Biochemical changes in the liver and gill of Cathorops spixii collected seasonally in two Brazilian estuaries under varying influences of anthropogenic activities

Autor(es)

Azevedo, J. S. ^[1] ; Braga, E. S. ^[1] ; Silva de Assis, H. C. ^[2] ; Oliveira Ribeiro, C. A. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Oceanog Inst, Sao Paulo - Brazil

^[2] Univ Fed Parana, Dept Farmacol, BR-81531990 Curitiba, Parana - Brazil

^[3] Univ Fed Parana, Dept Biol Celular, BR-81531990 Curitiba, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY; v. 96, p. 220-230, OCT 1 2013.
• Citações Web of Science: 8

Resumo

In order to understand environmental health by the use of a bioindicator species in estuaries, biochemical responses observed in the catfish Cathorops spixii such as catalase (CAT), glutathione-S-transferase (GST), lipid peroxidation (LPO) and acetylcholinesterase (AChE) were evaluated in liver and muscle. Furthermore, histological changes were also verified in liver and gills preparations. Fish were collected in three sites of the Santos-Sao Vicente estuary located at Sao Paulo (Brazil), subjected to varying levels of inputs of pollutants. For a reference site, specimens were sampled at Cananeia estuary at southern coast of Sao Paulo, a region with low anthropogenic influence. In general, no significant seasonal differences in antioxidant enzymes and lipid peroxidation responses were found in the organisms from the Cananeia estuary. However, in the polluted estuary (Santos-Sao Vicente), biochemical responses were observed by increases in GST hydroperoxides and decreases in AChE activities in the summer. Inhibition of AChE expression in fish from different areas of the Santos-Sao Vicente estuary in the summer was also found and can indicate neurotoxic effects in these organisms. Histopathological observation of gill and liver showed severe lesions, such as lamellar fusion and necrosis. (C) 2013 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 05/50769-2 - Materia organica na avaliacao de impacto ambiental no meio marinho: bioquimica, biogeoquimica, bioacumulacao e biotoxinas (mobio).
• Beneficiário: Elisabete de Santis Braga da Graça Saraiva
• Modalidade de apoio: Auxílio à Pesquisa - Regular