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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Modularity and evolutionary constraints in a baculovirus gene regulatory network

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Autor(es):
Oliveira, Juliana Velasco [1, 2] ; de Brito, Anderson Fernandes [1] ; Braconi, Carla Torres [1] ; de Melo Freire, Caio Cesar [1] ; Iamarino, Atila [1] ; de Andrade Zanotto, Paolo Marinho [1, 3]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Microbiol, Inst Biomed Sci ICB 2, Lab Mol Evolut & Bioinformat, Sao Paulo - Brazil
[2] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Ciencia & Tecnol Bioetanol CTBE, BR-13083970 Campinas, SP - Brazil
[3] Univ Sao Paulo, Dept Microbiol, Inst Ciencias Biomed ICB 2, Lab Evolucao Mol & Bioinformat, BR-05508900 Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: BMC SYSTEMS BIOLOGY; v. 7, SEP 4 2013.
Citações Web of Science: 9
Resumo

Background: The structure of regulatory networks remains an open question in our understanding of complex biological systems. Interactions during complete viral life cycles present unique opportunities to understand how host-parasite network take shape and behave. The Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) is a large double-stranded DNA virus, whose genome may encode for 152 open reading frames (ORFs). Here we present the analysis of the ordered cascade of the AgMNPV gene expression. Results: We observed an earlier onset of the expression than previously reported for other baculoviruses, especially for genes involved in DNA replication. Most ORFs were expressed at higher levels in a more permissive host cell line. Genes with more than one copy in the genome had distinct expression profiles, which could indicate the acquisition of new functionalities. The transcription gene regulatory network (GRN) for 149 ORFs had a modular topology comprising five communities of highly interconnected nodes that separated key genes that are functionally related on different communities, possibly maximizing redundancy and GRN robustness by compartmentalization of important functions. Core conserved functions showed expression synchronicity, distinct GRN features and significantly less genetic diversity, consistent with evolutionary constraints imposed in key elements of biological systems. This reduced genetic diversity also had a positive correlation with the importance of the gene in our estimated GRN, supporting a relationship between phylogenetic data of baculovirus genes and network features inferred from expression data. We also observed that gene arrangement in overlapping transcripts was conserved among related baculoviruses, suggesting a principle of genome organization. Conclusions: Albeit with a reduced number of nodes (149), the AgMNPV GRN had a topology and key characteristics similar to those observed in complex cellular organisms, which indicates that modularity may be a general feature of biological gene regulatory networks. (AU)

Processo FAPESP: 07/55282-0 - Estudo da rede genica do nucleopoliedrovirus anticarsia gemmatalis (agmnpv-2d) em linhagens de celulas de inseto distintas.
Beneficiário:Paolo Marinho de Andrade Zanotto
Linha de fomento: Auxílio à Pesquisa - Regular