Biochemical and microbiological characteristics of in situ biofilm formed on materials containing fluoride or amorphous calcium phosphate

Autor(es)

Ferreira, Lilian ^[1] ; Pedrini, Denise ^[1] ; Okamoto, Ana Claudia ^[1] ; Jardim Junior, Elerson Gaetti ^[1] ; Henriques, Tassia Araujo ^[1] ; Cannon, Mark ^[2] ; Botazzo Delbem, Alberto Carlos ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Aracatuba Sch Dent, Sao Paulo - Brazil

^[2] Northwestern Univ, Childrens Mem Hosp, Chicago, IL 60614 - USA

• Tipo de documento: Artigo Científico
• Fonte: AMERICAN JOURNAL OF DENTISTRY; v. 26, n. 4, p. 207-213, AUG 2013.
• Citações Web of Science: 6

Resumo

Purpose: To evaluate the biochemical and microbiological characteristics of in situ biofilm formed on materials that release fluoride (F-) or calcium (Ca++) and phosphate (Pi). Methods: This study comprised an in situ and in vitro experiment, utilizing three materials {[}Auralay XF and Fuji IX GP, containing fluoride, and Aegis containing amorphous calcium phosphate (ACP)] and bovine dental enamel slabs. For the in situ: 10 volunteers wore palatal devices, each containing four material specimens or enamel slabs that were treated with 20% sucrose solution. The biofilm had pH measurements on Day 7 and the composition was analyzed on Day 8 by assessing the following: F-, Ca++, Pi and insoluble extracellular polysaccharides (EPS) concentrations, and then identification of the microbiota. For the in vitro: materials/enamel were subjected to a 7-day pH-cycling regimen to determine F, Ca++ and Pi release. Results: The biofilm formed on F--releasing materials was richer in F, Ca++ and Pi and had lower mutans streptococci counts than enamel biofilm. The biofilm on the ACP-containing material exhibited similar Ca++ and Pi concentrations to biofilm on F--releasing materials. The materials showed buffering action compared with enamel. Biochemical and microbiological characteristics showed a less cariogenic biofilm on materials containing fluoride or amorphous calcium phosphate. (AU)

• Processo FAPESP: 08/02084-9 - Características bioquímicas e microbiológicas do biofilme dentário formado in situ sobre materiais que contêm flúor ou fosfato de cálcio amorfo.
• Beneficiário: Lilian Ferreira
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 08/06576-3 - Efeito de materiais utilizados como selantes de fóssulas e fissuras no biofilme dental formado in situ
• Beneficiário: Denise Pedrini
• Modalidade de apoio: Auxílio à Pesquisa - Regular