Ovarian blood vessel occlusion as a surgical sterilization method in rats

Autor(es)

Eduardo Murakami ^[1] ; Laíza Sartori de Camargo ^[2] ; Karym Christine de Freitas Cardoso ^[3] ; Marina Pacheco Miguel ^[4] ; Denise Cláudia Tavares ^[5] ; Cristiane dos Santos Honsho ^[6] ; Fabiana Ferreira de Souza ^[7]

Afiliação do(s) autor(es)

^[1] University of Franca - Brasil

^[2] UNIFRAN - Brasil

^[3] University of Franca - Brasil

^[4] Federal University of Goias - Brasil

^[5] Sao Paulo State University. School of Agrarian Sciences and Veterinary Medicine. Department of Preventive Veterinary Medicine and Animal, Reproduction - Brasil

^[6] UNIFRAN. Veterinary Surgery Division - Brasil

^[7] UNIFRAN. Animal Reproduction Division - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Acta Cirurgica Brasileira; v. 29, n. 4, p. 218-223, 2014-04-00.

Resumo

PURPOSE: To evaluate the female sterilization by occlusion of the ovarian blood flow, using the rat as experimental model.METHODS: Fifty-five females rats were divided into four groups: I (n=10), bilateral ovariectomy, euthanized at 60 or 90 days; II (n=5), opening the abdominal cavity, euthanized at 90 days; III (n=20), bilateral occlusion of the ovarian blood supply using titanium clips, euthanized at 60 or 90 days; and IV (n=20), bilateral occlusion of the ovarian blood supply using nylon thread, euthanized at 60 or 90 days. The estrous cycle was monitored by vaginal cytology. After euthanasia, the reproductive tissues were evaluated histologically.RESULTS: Ovarian atresia was identified macroscopically at 60 days after surgery in the rats in groups III and IV; however, most of the rats in group III maintained cyclicity. Histology of the tissues from group IV revealed that the ovarian tissue was replaced by dense fibrous connective tissue that was slightly vascularized and that intact follicles were absent by 90 days.CONCLUSION: Ovarian blood vessels occluded caused ischemia, leading to progressive tissue necrosis, and bilateral occlusion using a nylon ligature is a viable method for surgical sterilization. (AU)

• Processo FAPESP: 10/00657-1 - Avaliação da esterilização após a oclusão dos vasos sanguíneos ovarianos de ratas
• Beneficiário: Fabiana Ferreira de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/16314-9 - Avaliação da esterilização após a oclusão dos vasos sanguíneos ovarianos de ratas
• Beneficiário: Eduardo Murakami
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica