The effects of sleep deprivation on microRNA expression in rats submitted to pilocarpine-induced status epilepticus

Autor(es)

Matos, Gabriela ^[1] ; Scorza, Fulvio A. ^[2] ; Mazzotti, Diego R. ^[1] ; Guindalini, Camila ^[1] ; Cavalheiro, Esper A. ^[2] ; Tufik, Sergio ^[1] ; Andersen, Monica L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Psicobiol, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Expt Neurol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY; v. 51, p. 159-165, JUN 3 2014.
• Citações Web of Science: 8

Resumo

Epilepsy is a neurological disorder with significant prevalence and the individuals affected by this disease have a great probability of occurrence of a lethal phenomenon known as Sudden Unexpected Death in Epilepsy (SUDEP). SUDEP occurs mainly during the night and probably during sleep. The pathophysiological mechanisms involved in this lethal phenomenon are still obscure and new evidences that could corroborate in this area are warranted. Thus, the aim of the present study was to evaluate the effect of sleep deprivation in the expression of microRNA (miRNA) in the frontal cortex and heart tissues of adult male rats after 50 days of saline (SAL) or pilocarpine-induced status epilepticus (PILO). Initially 389 miRNA expressions were evaluated between SAL and PILO groups by microarray. Subsequently, 3 differentially expressed miRNAs of each tissue were investigated after total sleep deprivation (TSD 6 h) and paradoxical sleep deprivation (PSD 24 h). Still, it was analyzed that the effects of sleep rebound with equivalent duration of PSD and TSD. There was a significant increase of miR-146a expression, an important inflammatory modulator in the frontal cortex of PILO rats when compared to SAL animals. Animals treated with pilocarpine were affected by TSD (through overexpression of miRNAs related to inflammatory process) and these changes were maintained even after a sleep window of 6 h. In contrast, miRNAs associated with heart diseases were down-regulated in PSD rebound, suggesting a possible restoration of homeostasis in cardiovascular system of SAL and PILO groups. (C) 2014 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 12/05396-7 - Efeitos no desenvolvimento sensorial e sexual da prole de ratas submetidas à restrição de sono
• Beneficiário: Monica Levy Andersen
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/15110-8 - Sono e morte súbita nas epilepsias: provável base molecular?
• Beneficiário: Gabriela de Matos Barbosa Pimenta
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado