| Texto completo | |
| Autor(es): |
Mesquita Pasqualoto, Kerly Fernanda
[1]
;
Balan, Andrea
[2]
;
Barreto, Sandra Alves
[1]
;
Simons, Simone Michaela
[1]
;
Chudzinski-Tavassi, Ana Marisa
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Inst Butantan, Lab Bioquim & Biofis, BR-05503900 Sao Paulo - Brazil
[2] Brazilian Natl Lab Biosci, Ctr Struct & Mol Biol, BR-13084971 Campinas, SP - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PROTEIN AND PEPTIDE LETTERS; v. 21, n. 5, p. 452-457, MAY 2014. |
| Citações Web of Science: | 6 |
| Resumo | |
Specific blood coagulation inhibitors from hematophagous organisms, with different structures and novel mechanism of action, have been described and they represent promising agents for the treatment of a variety of human diseases related to coagulation and cancer. In our lab, the salivary glands transcriptome of the adult Amblyomma cajennense tick was previously characterized by expressed sequence tags (EST). A transcript that codes for a tissue factor pathway inhibitor (TFPI)-like protein with unique structure was found, and the recombinant form of this protein was named Amblyomin-X. This protein was able to inhibit the factor Xa amidolytic activity and the activation of factor X by the extrinsic tenase complex (FVIIa/TF). Herein, it was performed functional and structural evaluation of Amblyomin-X. The CD assay and molecular dynamics simulations revealed that Amblyomin-X is structurally stable and the naturally unfolded regions as well as the presence of three disulfide bridges in its Kunitz-type domain seem to sustain its inhibitory activity. Regarding the electrostatic potential mapping on the Kunitz-type region, the pattern of charged residues was not quite the same in comparison to human TFPI-1 and TFPI-2, pointing out there might be distinct functional and structural features, which are going to be experimentally exploited. (AU) | |
| Processo FAPESP: | 10/52669-3 - Avaliação do mecanismo de ação pró-apoptótica do Amblyomin-X |
| Beneficiário: | Ana Marisa Chudzinski-Tavassi |
| Linha de fomento: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 11/21912-2 - Avaliação do mecanismo de ação pró-apoptótica do Amblyomin-X |
| Beneficiário: | Kerly Fernanda Mesquita Pasqualoto |
| Linha de fomento: | Bolsas no Brasil - Programa Capacitação - Treinamento Técnico |