Hepatic ischemic preconditioning increases portal vein flow in experimental liver ischemia reperfusion injury

Autor(es)

Figueira, Estela R. R. ^[1] ; Rocha-Filho, Joel A. ^[2] ; Nakatani, Mauro ^[3] ; Buto, Marcelo F. S. ^[3] ; Tatebe, Eduardo R. ^[3] ; Andre, Vitor ^[3] ; Cecconello, Ivan ^[1] ; D'Albuquerque, Luiz A. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Hosp Clin, Dept Gastroenterol, Lab Med Invest LIM37 Discipline Liver & Gastroent, BR-01329000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Hosp Clin, Discipline Anesthesiol, BR-01329000 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Hosp Clin, Sch Med, BR-01329000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Hepatobiliary & Pancreatic Diseases International; v. 13, n. 1, p. 40-47, FEB 15 2014.
• Citações Web of Science: 6

Resumo

BACKGROUND: Ischemic preconditioning (IPC) has been shown to decrease liver injury and to increase hepatic microvascular perfusion after liver ischemia reperfusion. This study aimed to evaluate the effects of IPC on hemodynamics of the portal venous system. METHODS: Thirty-two rats were randomized into two groups: IPC group and control group. The rats of the IPC group underwent IPC by 10 minutes of liver ischemia followed by 10 minutes of reperfusion before liver ischemia, and the rats of the control group were subjected to 60 minutes of partial liver ischemia. Non-ischemic lobes were resected immediately after reperfusion. The animals were studied at 4 hours and 12 hours after reperfusion. Mean arterial pressure, heart rate, portal vein flow and pressure were analyzed. Blood was collected for the determination of the levels of aspartate aminotransferase, alanine aminotransferase, calcium, lactate, pH, bicarbonate, and base excess. RESULTS: IPC increased the mean portal vein flow at 4 hours and 12 hours after reperfusion. IPC recovered 78% of the mean portal vein flow at 12 hours after reperfusion. IPC decreased the levels of aspartate aminotransferase, alanine aminotransferase and lactate, and increased the levels of ionized calcium, bicarbonate and base excess at 12 hours after reperfusion. CONCLUSIONS: This study demonstrated that IPC increases portal vein flow and enhances hepatoprotective effects in liver ischemia reperfusion. The better recovery of portal vein flow after IPC may be correlated with the lower levels of transaminases and with the better metabolic profile. (AU)

• Processo FAPESP: 11/05214-3 - Efeitos do pré-condicionamento isquêmico na hemodinâmica e no metabolismo hepático em modelo experimental de isquemia/reperfusão do fígado em ratos
• Beneficiário: Estela Regina Ramos Figueira
• Modalidade de apoio: Auxílio à Pesquisa - Regular