A Multivariate Calibration Procedure for UV/VIS Spectrometric Monitoring of BHK-21 Cell Metabolism and Growth

Autor(es):Mostrar menos -

Leme, Jaci ^[1] ; Fernandez Nunez, Eutimio Gustavo ^{[2, 3]} ; Parizotto, Leticia de Almeida ^[2] ; Chagas, Wagner Antonio ^[1] ; dos Santos, Erica Salla ^[1] ; Prestia Caricati, Aline Tojeira ^[1] ; de Rezende, Alexandre Goncalves ^[4] ; Vale da Costa, Bruno Labate ^{[2, 5]} ; Ventini Monteiro, Daniela Cristina ^[4] ; Lopes Boldorini, Vera Lucia ^[4] ; Calil Jorge, Soraia Attie ^[4] ; Astray, Renato Mancini ^[4] ; Pereira, Carlos Augusto ^{[4, 2]} ; Caricati, Celso Pereira ^[1] ; Tonso, Aldo ^[2]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Especial Pesquisa & Desenvolvimento Imunol V, BR-05503900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Escola Politecn, Dept Engn Quim, Lab Celulas Anim, BR-05508900 Sao Paulo - Brazil

^[3] Univ Estadual Paulista, Dept Ciencias Biol, BR-19806900 Assis, SP - Brazil

^[4] Inst Butantan, Lab Imunol Viral, BR-05503900 Sao Paulo - Brazil

^[5] Inst Pesquisas Tecnol Estado Sao Paulo SA, Nucleo Bionanomanufatura, Lab Biotecnol Ind, BR-05508901 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOTECHNOLOGY PROGRESS; v. 30, n. 1, p. 241-248, JAN 2014.
• Citações Web of Science: 5

Resumo

Monitoring mammalian cell culture with UV-vis spectroscopy has not been widely explored. The aim of this work was to calibrate Partial Least Squares (PLS) models from off-line UV-vis spectral data in order to predict some nutrients and metabolites, as well as viable cell concentrations for mammalian cell bioprocess using phenol red in culture medium. The BHK-21 cell line was used as a mammalian cell model. Spectra of samples taken from batches performed at different dissolved oxygen concentrations (10, 30, 50, and 70% air saturation), in two bioreactor configurations and with two strategies to control pH were used to calibrate and validate PLS models. Glutamine, glutamate, glucose, and lactate concentrations were suitably predicted by means of this strategy. Especially for glutamine and glucose concentrations, the prediction error averages were lower than 0.50 +/- 0.10 mM and 2.21 +/- 0.16 mM, respectively. These values are comparable with those previously reported using near infrared and Raman spectroscopy in conjunction with PLS. However, viable cell concentration models need to be improved. The present work allows for UV-vis at-line sensor development, decrease cost related to nutrients and metabolite quantifications and establishment of fed-batch feeding schemes. (c) 2013 American Institute of Chemical Engineers Biotechnol. Prog., 30:241-248, 2014 (AU)

• Processo FAPESP: 10/52521-6 - Producao de glicoproteinas da raiva atraves do cultivo de celulas bhk-21 em biorreatores usando sistema de expressao semliki forest virus (sfv).
• Beneficiário: Eutimio Gustavo Fernández Núñez
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado