Alterations of C-MYC, NKX3.1, and E-Cadherin Expression in Canine Prostate Carcinogenesis

Autor(es)

Fonseca-Alves, Carlos E. ^[1] ; Rodrigues, Marcela M. P. ^[2] ; de Moura, Veridiana M. B. D. ^[3] ; Rogatto, Silvia R. ^{[4, 5]} ; Laufer-Amorim, Renee ^[1]

Afiliação do(s) autor(es)

^[1] Sch Vet Med & Anim Sci, Dept Vet Clin, Botucatu, SP - Brazil

^[2] Botucatu Med Sch, Dept Anesthesiol, Botucatu, SP - Brazil

^[3] Univ Fed Goias, Sch Vet & Zootechny, Dept Vet Med, Goiania, Go - Brazil

^[4] Botucatu Med Sch, Dept Urol, Botucatu, SP - Brazil

^[5] AC Camargo Hosp, Int Ctr Res CIPE, Liberdade, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROSCOPY RESEARCH AND TECHNIQUE; v. 76, n. 12, p. 1250-1256, DEC 2013.
• Citações Web of Science: 17

Resumo

The dog (canis lupus familiaris) is the only other species besides humans that develop spontaneous prostatic carcinomas (PCa) at a high frequency. The canine model is primarily utilized for the study of the PCa molecular mechanisms and provides a natural animal model for the study of potential therapies. In humans, the PCa frequently exhibits mutations in the C-MYC and a reduced expression of the E-cadherin and NKX3.1 proteins. This study's objective was to evaluate the NKX3.1, C-MYC, and E-cadherin expression in the canine normal prostate, benign prostatic hyperplasia (BPH), proliferative inflammatory atrophy (PIA) and PCa and to verify differences in expression and subcellular localization of these proteins in the prostatic carcinogenesis. A tissue microarray (TMA) slide was constructed, and immunohistochemistry with antibodies raised against C-MYC, NKX3.1, E-cadherin and p63 was performed using the peroxidase and DAB methods. The C-MYC protein expression was elevated in the cytoplasm and nuclei of the canine PCa and PIA compared with the normal prostate (P=0.004. The NKX3.1 protein expression was reduced in 94.75% of the PCa and 100% of the PIA compared with the normal prostate (P=0.0022). In fact, the expression of E-cadherin trended towards a decrease in carcinomas when compared to normal prostate and PIA. By immunohistochemistry, more p63-positive basal cells were observed in the PCa and PIA when compared with the normal prostate (P=0.0002). This study has demonstrated that the carcinogenesis of canine prostatic tissue may be related to basal cell proliferation, the gain of C-MYC function and the loss of NKX3.1 protein expression. Microsc. Res. Tech. 76:1250-1256, 2013. (c) 2013 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 10/13774-6 - Expressão do c-Myc e NKX3.1 por imunoistoquímica em microarranjo de tecido (TMA) de lesões pré-neoplásicas e neoplásicas na próstata de cães
• Beneficiário: Carlos Eduardo Fonseca Alves
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 12/16068-0 - Avaliação epigenética dos genes NKX3.1 e CDH1 e expressão do c-myc, NKX3.1 e caderina-E por imunoistoquímica em microarranjo de tecido (TMA) de lesões pré-neoplásicas e neoplásicas na próstata de cães
• Beneficiário: Renee Laufer Amorim
• Linha de fomento: Auxílio à Pesquisa - Regular