Alterations of C-MYC, NKX3.1, and E-Cadherin Expression in Canine Prostate Carcinogenesis

Author(s)

Fonseca-Alves, Carlos E. ^[1] ; Rodrigues, Marcela M. P. ^[2] ; de Moura, Veridiana M. B. D. ^[3] ; Rogatto, Silvia R. ^{[4, 5]} ; Laufer-Amorim, Renee ^[1]

Affiliation

^[1] Sch Vet Med & Anim Sci, Dept Vet Clin, Botucatu, SP - Brazil

^[2] Botucatu Med Sch, Dept Anesthesiol, Botucatu, SP - Brazil

^[3] Univ Fed Goias, Sch Vet & Zootechny, Dept Vet Med, Goiania, Go - Brazil

^[4] Botucatu Med Sch, Dept Urol, Botucatu, SP - Brazil

^[5] AC Camargo Hosp, Int Ctr Res CIPE, Liberdade, SP - Brazil

• Document type: Journal article
• Source: MICROSCOPY RESEARCH AND TECHNIQUE; v. 76, n. 12, p. 1250-1256, DEC 2013.
• Web of Science Citations: 15

Abstract

The dog (canis lupus familiaris) is the only other species besides humans that develop spontaneous prostatic carcinomas (PCa) at a high frequency. The canine model is primarily utilized for the study of the PCa molecular mechanisms and provides a natural animal model for the study of potential therapies. In humans, the PCa frequently exhibits mutations in the C-MYC and a reduced expression of the E-cadherin and NKX3.1 proteins. This study's objective was to evaluate the NKX3.1, C-MYC, and E-cadherin expression in the canine normal prostate, benign prostatic hyperplasia (BPH), proliferative inflammatory atrophy (PIA) and PCa and to verify differences in expression and subcellular localization of these proteins in the prostatic carcinogenesis. A tissue microarray (TMA) slide was constructed, and immunohistochemistry with antibodies raised against C-MYC, NKX3.1, E-cadherin and p63 was performed using the peroxidase and DAB methods. The C-MYC protein expression was elevated in the cytoplasm and nuclei of the canine PCa and PIA compared with the normal prostate (P=0.004. The NKX3.1 protein expression was reduced in 94.75% of the PCa and 100% of the PIA compared with the normal prostate (P=0.0022). In fact, the expression of E-cadherin trended towards a decrease in carcinomas when compared to normal prostate and PIA. By immunohistochemistry, more p63-positive basal cells were observed in the PCa and PIA when compared with the normal prostate (P=0.0002). This study has demonstrated that the carcinogenesis of canine prostatic tissue may be related to basal cell proliferation, the gain of C-MYC function and the loss of NKX3.1 protein expression. Microsc. Res. Tech. 76:1250-1256, 2013. (c) 2013 Wiley Periodicals, Inc. (AU)

• FAPESP's process: 12/16068-0 - Epigenetic evaluation of NKX3.1 and CDH1 and immunohistochemistry expression of c-myc, NKX3.1 and E-cadherin on tissue microarray (TMA) of pre-neoplastic and neoplastic prostate of dogs
• Grantee: Renee Laufer Amorim
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/13774-6 - Imunohistochemistry evaluation of C-MYC and NKX3.1 in tissue microarray (TMA) in pre-neoplastic and neoplastic lesions in canine prostate.
• Grantee: Carlos Eduardo Fonseca Alves
• Support Opportunities: Scholarships in Brazil - Master