The enzymatic recalcitrance of internodes of sugar cane hybrids with contrasting lignin contents

Autor(es)

Costa, Thales H. F. ^[1] ; Masarin, Fernando ^[1] ; Bonifacio, Taina O. ^[1] ; Milagres, Adriane M. F. ^[1] ; Ferraz, Andre ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Escola Engn Lorena, Dept Biotechnol, BR-12602810 Lorena, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INDUSTRIAL CROPS AND PRODUCTS; v. 51, p. 202-211, NOV 2013.
• Citações Web of Science: 23

Resumo

The recalcitrance in grasses varies according to cell type and maturation. The origin of the recalcitrance in different regions from sugar cane internodes with varied lignin contents was evaluated. The efficiency of enzymatic hydrolysis was correlated with the chemical, micromorphological and microspectrophotometric characteristics of the samples. The internodes of three sugar cane hybrids were dissected into four different fractions. The outermost fraction and the rind were the most recalcitrant regions, whereas the pith-rind interface and the pith were less recalcitrant. Cellulose conversion reached 86% after 72 h of enzymatic digestion of the pith from the hybrid with the lowest lignin content. There was an inversely proportional correlation between the area occupied by vascular bundles and the efficiency of cellulose hydrolysis. High cellulose and low lignin or hemicellulose contents enhanced the efficiency of enzymatic hydrolysis of the polysaccharides. The critical evaluation of the results permitted to propose an empirical parameter for predicting cellulose conversion levels that accounts for the positive effect of high cellulose and low lignin plus hemicellulose and the detrimental effect of abundant vascular bundles. The cellulose conversion levels fit well to this calculated parameter, following a second order polynomial with an r(2) value of 0.96. (C) 2013 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 10/50051-2 - Propriedades fisico-quimicas e distribuicao topoquimica da lignina em dez diferentes hibridos de cana-de-acucar e sua correlacao com a eficiencia num processo de hidrolise enzimatica da fracao polissaca...
• Beneficiário: Fernando Masarin
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/50535-2 - Análise micro-espectrofotométrica aplicada ao estudo da recalcitrância em materiais lignocelulósicos
• Beneficiário: Andre Luis Ferraz
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/56256-5 - Topochemistry, porosity and chemical composition determining successful enzymatic sacarification of sugarcane bagasse
• Beneficiário: Adriane Maria Ferreira Milagres
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático