In Vitro Antimicrobial Activity of Plant-Derived Diterpenes against Bovine Mastitis Bacteria

Autor(es)

Fonseca, Ariana P. ^[1] ; Estrela, Fernanda T. ^[1] ; Moraes, Thais S. ^[1] ; Carneiro, Luiza J. ^[1] ; Bastos, Jairo K. ^[2] ; dos Santos, Raquel A. ^[1] ; Ambrosio, Sergio R. ^[1] ; Martins, Carlos H. G. ^[1] ; Veneziani, Rodrigo C. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Franca, Nucleus Res Sci & Technol, BR-1404600 Franca - Brazil

^[2] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Lab Pharmacognosy, BR-14040903 Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecules; v. 18, n. 7, p. 7865-7872, JUL 2013.
• Citações Web of Science: 10

Resumo

We evaluated the antibacterial activity of three diterpenes isolated from natural sources against a panel of microorganisms responsible for bovine mastitis. ent-Copalic acid (CA) was the most active metabolite, with promising MIC values (from 1.56 to 6.25 mu g mL(-1)) against Staphylococcus aureus (ATCC and clinical isolate), Staphylococcus epidermidis, Streptococcus agalactiae, and Streptococcus dysgalactiae. We conducted time-kill assays of CA against S. aureus, a commensal organism considered to be a ubiquitous etiological agent of bovine mastitis in dairy farms worldwide. In the first 12 h, CA only inhibited the growth of the inoculums (bacteriostatic effect), but its bactericidal effect was clearly noted thereafter (between 12 and 24 h). In conclusion, CA should be considered for the control of several Gram-positive bacteria related to bovine mastitis. (AU)

• Processo FAPESP: 11/13630-7 - Validação química e farmacológica de extratos e princípios ativos de espécies de Copaifera
• Beneficiário: Jairo Kenupp Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Temático