The oral administration of trans-caryophyllene attenuates acute and chronic pain in mice

Autor(es)

Paula-Freire, L. I. G. ^[1] ; Andersen, M. L. ^[1] ; Gama, V. S. ^[2] ; Molska, G. R. ^[1] ; Carlini, E. L. A. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Psicobiol, Sao Paulo - Brazil

^[2] Univ Sao Judas Tadeu, Fac Farm, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Med Prevent, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Phytomedicine; v. 21, n. 3, p. 356-362, FEB 15 2014.
• Citações Web of Science: 36

Resumo

Trans-caryophyllene is a sesquiterpene present in many medicinal plants' essential oils, such as Ocimum gratissimum and Cannabis sativa. In this study, we evaluated the antinociceptive activity of transcaryophyllene in murine models of acute and chronic pain and the involvement of trans-caryophyllene in the opioid and endocannabinoid systems. Acute pain was determined using the hot plate test (thermal nociception) and the formalin test (inflammatory pain). The chronic constriction injury (CCI) of the sciatic nerve induced hypernociception was measured by the hot plate and von Frey tests. To elucidate the mechanism of action, mice were pre-treated with naloxone or AM630 30 min before the trans-caryophyllene treatment. Afterwards, thermal nociception was evaluated. The levels of IL-1 beta were measured in CCI-mice by ELISA. Trans-caryophyllene administration significantly minimized the pain in both the acute and chronic pain models. The antinociceptive effect observed during the hot plate test was reversed by naloxone and AM630, indicating the participation of both the opioid and endocannabinoid system. Trans-caryophyllene treatment also decreased the IL-1 beta levels. These results demonstrate that transcaryophyllene reduced both acute and chronic pain in mice, which may be mediated through the opioid and endocannabinoid systems. (C) 2013 Elsevier GmbH. All rights reserved. (AU)

• Processo FAPESP: 09/51881-1 - Avaliacao do efeito analgesico e antialodinico de ocimum gratissimum l. e seus principios ativos isolados em um modelo de dor neuropatica em camundongos
• Beneficiário: Lyvia Izaura Gomes de Paula Freire
• Modalidade de apoio: Bolsas no Brasil - Doutorado