Pretreatment of sugarcane bagasse with microwaves irradiation and its effects on the structure and on enzymatic hydrolysis

Autor(es)

de Souza Moretti, Marcia Maria ^[1] ; Bocchini-Martins, Daniela Alonso ^[2] ; Carreira Nunes, Christiane da Costa ^[1] ; Villena, Maria Arevalo ^[3] ; Perrone, Olavo Micali ^[1] ; da Silva, Roberto ^[1] ; Boscolo, Mauricio ^[1] ; Gomes, Eleni ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, IBILCE, BR-15054000 Sao Paulo - Brazil

^[2] Sao Paulo State Univ, UNESP, IQ, BR-15054000 Sao Paulo - Brazil

^[3] Univ Castilla La Mancha, E-13071 Ciudad Real - Spain

• Tipo de documento: Artigo Científico
• Fonte: APPLIED ENERGY; v. 122, p. 189-195, JUN 1 2014.
• Citações Web of Science: 67
• Assunto(s): Bioenergia Etanol Hidrólise enzimática Bagaço de cana-de-açúcar

Resumo

This paper refers to the new proposal of pre-treatment of sugarcane bagasse with microwave associated to glycerol, seeking greater release of fermentable sugars during enzymatic hydrolysis. The residue was subjected to microwave irradiation for 5 min with distilled water, phosphoric acid (pH 3.0) and glycerol (100%) before being enzymatically hydrolyzed using cellulase enzyme extract Myceliophthora thermophila M.7.7. and the commercial enzyme cocktail Celluclast 1.5 LA variety of analyses including measurement of BET surface analysis, MET, TGA, DTG, DSC, ATR-FTIR and PAD-HPLC were used to facilitate the understanding of the physical and chemical characteristics of the solid fraction resulting from pre-treatment. Infrared spectra of untreated and treated bagasse in microwave irradiation and glycerol showed significant differences in the regions 1635, 1600 and 1510 related vibration of the aromatic ring, and the band at 1100 cm(-1) is attributed to an overlap of C-O-H elongation of primary and secondary alcohols and at 980 cm(-1) to stretching of glycosidic linkages C-O-C. The thermal analysis showed that the bagasse treated in a microwave irradiation and glycerol has higher thermal stability compared to the untreated bagasse. The experimental results indicated that 5.4 and 11.3% w/w of lignin and xylan fractions, respectively, are degraded after pretreatment of bagasse in microwave heating with glycerol. The highest yields of hydrolysis of hemicellulose (22.4%) and cellulose (40.2%) w/w were obtained in the reaction mixture containing the enzyme and Celluclast commercial cane bagasse treated in a microwave irradiation and glycerol after 24 h of incubation. The association of microwave and glycerol is a new alternative to deconstruction of lignocellulose structure. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 14/02080-4 - Produção de etanol de bagaço de cana-de-açúcar pré-tratado com ozônio: estudo de inibidores, fermentação e leveduras industriais
• Beneficiário: Roberto da Silva
• Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 10/12624-0 - Aplicação de métodos físico-químicos e enzimáticos na sacarificação do bagaço de cana: estudos de microrganismos, processos fermentativos relacionados e métodos de hidrólise
• Beneficiário: Eleni Gomes
• Linha de fomento: Auxílio à Pesquisa - Temático