Multivariate Analysis of the Stability of Spray-Dried Eupenicillium javanicum Peptidases

Autor(es)

Hamin Neto, Youssef Ali Abou ^[1] ; Pedro de Freitas, Luis Alexandre ^[1] ; Cabral, Hamilton ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Drying Technology; v. 32, n. 5, p. 614-621, APR 4 2014.
• Citações Web of Science: 5

Resumo

Peptidases occupy a central position in the enzyme market because of their importance in many areas, such as for physiological processes, foods, and detergents, as well as in the pharmaceutical, leather, and biotechnology industries. Microbial production is among the major sources of peptidases because it presents many advantages when compared with other methods. In this study, the metallopeptidases produced by the fungus Eupenicillium javanicum under a solid-state fermentation bioprocess were spray-dried. The enzymatic extract was dried using drying adjuvants, and optimal conditions for preserving enzymatic activity were studied following a Box-Behnken experimental design. The spray process factors studied were the air-drying temperature, enzyme feed flow rate, and the proportion of enzyme/additive. The responses analyzed were the dry extract yield and enzymatic activity after spray drying. Additionally, the stability of the dry extracts was assessed during 180 days at 4 degrees C and 25 degrees C. The results revealed extract yields of up to 66.12% and good enzymatic activity for intermediate values of temperature and adjuvant proportions. Furthermore, the dried enzymatic extracts showed potential for future commercial applications because of their stability at 25 degrees C for 180 days. (AU)

• Processo FAPESP: 11/06986-0 - Determinação da especificidade de peptidases isoladas de fungos usando peptídeos FRET como substratos
• Beneficiário: Hamilton Cabral
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/20872-7 - Desenvolvimento e aplicação da técnica de nebulização-liofilização (spray freeze drying) para produtos fitofarmacêuticos
• Beneficiário: Luis Alexandre Pedro de Freitas
• Modalidade de apoio: Auxílio à Pesquisa - Regular