Fucoxanthin in association with Vitamin c acts as modulators of human neutrophil function

Autor(es)

Morandi, A. C. ^[1] ; Molina, N. ^[1] ; Guerra, B. A. ^[1] ; Bolin, A. P. ^[1] ; Otton, R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Cruzeiro Sul, Postgrad Program, CBS, BR-03342000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: EUROPEAN JOURNAL OF NUTRITION; v. 53, n. 3, p. 779-792, APR 2014.
• Citações Web of Science: 8

Resumo

Neutrophils provide the first line of defense of the innate immune system by phagocytosing, killing and digesting bacteria and fungi. During this process, neutrophils produce reactive oxygen species (ROS), which in excess, can damage the cells themselves and surrounding tissues. The carotenoid fucoxanthin (Fc) has been studied concerning its antioxidant and anti-inflammatory actions. Vitamin c (Vc) also demonstrates potent antioxidant action. This study aimed to evaluate the effect of Fc (2 mu M) in association with Vc (100 mu M) on functional parameters of human neutrophils in vitro. We evaluated the migration and phagocytic capacity, intracellular calcium mobilization, ROS production (O (2) (center dot-) , H2O2, HOCl), myeloperoxidase activity, profile of antioxidant enzymes, phosphorylation of p38 MAPK and p65 NF kappa B subunit, GSH/GSSG ratio and release of pro-inflammatory cytokines (TNF-alpha and IL-6) in neutrophils under different stimuli. We verified an increase in phagocytic capacity for all treatments, together with an increase in intracellular calcium only in cells treated with Fc and Fc + Vc. ROS production was reduced by all treatments, although Vc was a better antioxidant than Fc. Phosphorylation of the p-65 subunit of NF kappa B was reduced in cells treated with Fc + Vc and release of TNF-alpha and IL-6 was reduced by all treatments. These findings indicate that the regulation of inflammatory cytokines by neutrophils is not exclusively under the control of the NF kappa B pathway. Fc reduced the activity of some antioxidant enzymes, whereas Vc increased GR activity and the GSH/GSSG ratio. In conclusion, the results presented in this study clearly show an immunomodulatory effect of the carotenoid fc alone or in combination with Vc on the function of human neutrophils. (AU)

• Processo FAPESP: 11/00880-5 - Efeito do carotenóide fucoxantina associado à vitamina C na função de neutrófilos humanos in vitro
• Beneficiário: Ana Carolina Morandi
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica