Host-biting rate and susceptibility of some suspected vectors to Leishmania braziliensis

Autor(es)

de Souza Leal Diniz, Morgana Michele Cavalcanti ^[1] ; Ovallos, Fredy Galvis ^[1] ; de Castro Gomes, Claudia Maria ^[2] ; Lavitschka, Cecilia de Oliveira ^[3] ; Bianchi Galati, Eunice Aparecida ^[4]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Publ Hlth, Postgrad Program Publ Hlth, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med, Dept Pathol, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Publ Hlth, PAP FUNDAP Dept Epidemiol, Profess Improvement Program, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Publ Hlth, Dept Epidemiol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PARASITES & VECTORS; v. 7, MAR 31 2014.
• Citações Web of Science: 8

Resumo

Background: American tegumentary leishmaniasis is a serious Brazilian public health problem. This diseases is attributed to seven species of Leishmania, however, the majority of cases are associated with Leishmania braziliensis. Some phlebotomine species have been implicated in the transmission of this parasite, nonetheless only Psychodopygus wellcomei has had its vectorial competence demonstrated. Thus this study sought to assess some parameters related to the vectorial capacity of anthropophilic species of sand fly occurring in Sao Paulo state: Pintomyia fischeri, Migonemyia migonei Nyssomyia intermedia, Nyssomyia whitmani, Expapillata firmatoi and Psychodopygus ayrozai, under laboratory conditions. These parameters were the duration of the gonotrophic cycle, proportion of females which feed on hamster, the rate of infection by L. braziliensis and the duration of the extrinsic incubation period. Methods: The sandflies were collected in three regions of the Sao Paulo state: Greater Sao Paulo and the Mogi Guacu and Iporanga municipalities. To assess the proportion of engorged females the insects were fed on hamsters to estimate the duration of the gonotrophic cycle. To estimate the susceptibility to infection of each species, their females were fed on hamsters infected with Leishmania braziliensis and dissected to ascertain the localization of the flagellates and estimate the extrinsic incubation period. Results: Low hamster attractiveness to Ps. ayrozai was observed. A high proportion of engorged females was observed when the hamster had its whole body exposed. The gonotrophic cycle ranged between three and eight days. Mg. migonei, Pi. fischeri, Ny. neivai, Ny. intermedia, Ny. whitmani and Ex.firmatoi presented susceptibility to infection by L. braziliensis. The highest infection rate (34.4%) was observed for Ny. whitmani and the lowest for Ny. intermedia (6.6%). Mg. migonei presented late-stage infection forms on the fifth day after feeding, but in the other species these forms were observed as from the fourth day. Conclusions: Our results, together with other parameters of their behavior under natural conditions, suggest the potential role of Ex. firmatoi as vector of this parasite and reinforce that of Mg. migonei, Pi. fischeri, Ny. neivai, Ny. intermedia and Ny. whitmani in the areas in which they occur. (AU)

• Processo FAPESP: 09/53414-1 - Estudo da capacidade vetorial de Migonemyia migonei (França) e de Pintomyia fischeri (Pinto) (Diptera: Psychodidae) para Leishmania (Leishmania) infantum chagasi Cunha & Chagas e Leishmania (Viannia) braziliensis Vianna
• Beneficiário: Eunice Aparecida Bianchi Galati
• Modalidade de apoio: Auxílio à Pesquisa - Regular