Homogenous HIV-1 subtype B quasispecies in Brazilian men and women recently infected via heterosexual transmission

Autor(es)

Gouveia, Nancy Lima ^[1] ; Camargo, Michelle ^[1] ; Caseiro, Marcos Montani ^[2] ; Ramos Janini, Luiz Mario ^[1] ; Araripe Sucupira, Maria Cecilia ^[1] ; Diaz, Ricardo Sobhie ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Paulista Sch Med, Sao Paulo - Brazil

^[2] Ctr Univ Lusiada, Santos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VIRUS GENES; v. 48, n. 3, p. 421-428, JUN 2014.
• Citações Web of Science: 1

Resumo

HIV has extraordinary genetic mutability, both among individuals and at the population level. However, studies of primary HIV-1 infection and serum-converters indicate that the viral population is homogeneous at the sequence level, which suggests clonal HIV transmission. It remains unclear whether this feature applies to the female population. Ten single genome amplification sequences were generated from ten individuals (five females) with recent heterosexually acquired HIV infection as determined by the serologic testing algorithm for recent HIV seroconversion. Intra-individual genetic diversity was equally low in both genders (< 2 %), with mean and median variations of 0.8 and 0 %, respectively. All of the subjects were infected with clade B. Three subjects (two females) appeared to be infected by two related viral populations, and four subjects harbored non-R5 strains. Our results support the hypothesis of clonal selection for sexual transmission of HIV-1 in both genders. Future studies that generate a larger number of clones, preferably by next generation deep sequencing, are needed to confirm these results. (AU)

• Processo FAPESP: 98/14381-4 - Determinação da prevalência de resistência genotípica e da diversidade genética em casos de infecção recente pelo Vírus da Imunodeficiência Humana (HIV-1)
• Beneficiário: Ricardo Sobhie Diaz
• Modalidade de apoio: Auxílio à Pesquisa - Temático