Platelet-Rich Plasma, Low-Level Laser Therapy, or Their Combination Promotes Periodontal Regeneration in Fenestration Defects: A Preliminary In Vivo Study

Autor(es):Mostrar menos -

Nagata, Maria J. H. ^[1] ; de Campos, Natalia ^[1] ; Messora, Michel R. ^[2] ; Pola, Natalia M. ^[1] ; Santinoni, Carolina S. ^[1] ; Bomfim, Suely R. M. ^[3] ; Fucini, Stephen E. ^[1] ; Ervolino, Edilson ^[4] ; de Almeida, Juliano M. ^[1] ; Theodoro, Leticia H. ^[1] ; Garcia, Valdir G. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Dent Sch Aracatuba, Dept Surg & Integrated Clin, Aracatuba, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Sch Dent, Dept Oral & Maxillofacial Surg & Traumatol, USP, Ribeirao Preto, SP - Brazil

^[3] Sao Paulo State Univ, Vet Sch Aracatuba, Dept Clin Surg & Anim Reprod, Sao Paulo - Brazil

^[4] Sao Paulo State Univ, UNESP, Dept Basic Sci, Dent Sch Aracatuba, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Periodontology; v. 85, n. 6, p. 770-778, JUN 2014.
• Citações Web of Science: 8

Resumo

Background: This study histomorphometrically analyzes the influence of platelet- rich plasma (PRP), low- level laser therapy (LLLT), or their combination on the healing of periodontal fenestration defects (PFDs) in rats. Methods: PFDs were surgically created in the mandibles of 80 rats. The animals were randomly divided into four groups: 1) C (control) and 2) PRP, defects were filled with blood clot or PRP, respectively; 3) LLLT and 4) PRP/LLLT, defects received laser irradiation, were filled with blood clot or PRP, respectively, and then irradiated again. Animals were euthanized at either 10 or 30 days post-surgery. Percentage of new bone (NB), density of newly formed bone (DNB), new cementum (NC), and extension of remaining defect (ERD) were histomorphometrically evaluated. Data were statistically analyzed (analysis of variance; Tukey test, P < 0.05). Results: At 10 days, group PRP presented ERD significantly lower than group C. At 30 days, group PRP presented NB and DNB significantly greater than group C. Groups LLLT, PRP, and PRP/LLLT showed significant NC formation at 30 days, with collagen fibers inserted obliquely or perpendicularly to the root surface. NC formation was not observed in any group C specimen. Conclusions: LLLT, PRP, or their combination all promoted NC formation with a functional periodontal ligament. The combination PRP/LLLT did not show additional positive effects compared to the use of either therapy alone. (AU)

• Processo FAPESP: 08/52608-4 - Avaliacao da influencia do plasma rico em plaquetas e do laser de baixa intensidade na cicatrizacao de defeitos de fenestracao periodontal em ratos. estudo histologico e histometrico.
• Beneficiário: Natália de Campos Kajimoto
• Modalidade de apoio: Bolsas no Brasil - Mestrado